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  • 1
    ISSN: 1432-1335
    Keywords: Ehrlich ascites tumor cells ; DNA over-replication ; Anaerobiosis ; G2M phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary G2-enriched fractions of Ehrlich ascites tumor cells (up to 80%–85% G2 cells) separated from anaerobically and aerobically cultured asynchronous populations by centrifugal elutriation revealed the same growth characteristics after recultivation under standard conditions: a significant proportion of cells with increased DNA (DNA content〉4C) emerged. Interruption of DNA synthesis by deprivation of oxygen may account for polyploidisation (over-replication) of DNA but other mechanisms must be taken into consideration.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1335
    Keywords: Ehrlich ascites tumor cells ; Methylglyoxal ; Glucosone ; Galactosone ; Growth inhibition ; DNA synthesis ; Protein Synthesis ; Energy Metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Proliferation of in vitro grown Ehrlich ascites tumor cells is completely inhibited by 0.2–0.4 mM methylglyoxal and 1–2mM glucosone or galactosone without severely affecting viability (dye exclusion test); no phase-specific arrest of cell growth is observed. 2. Incorporation of [14C] thymidine into the acid-insoluble fraction of the cells decreases within a few minutes to less than 50% of that in controls in the presence of 0.4 mM methylglyoxal, and 2 mM glucosone or galactosone causes a comparable inhibition of DNA synthesis after 2 h or 4 h, respectively. 3. The action of 0.4 mM methylglyoxal inhibits incorporation of [14C] leucine within a few minutes by more than 70%, while 2 mM glucosone and galactosone are significantly less effective (50%–60% inhibition after 12 h). 4. While methylglyoxal and galactosone do not severely affect lactate production of the cells, 2 mM glucosone reduces glycolysis by 60%–70%; ATP/ADP ratios did not fall below 3.5 in the presence of the inhibitors (controls 4–6). 5. It is suggested that the reaction potentialities of the oxaldehyde function of the inhibitors play an important role in their growth-inhibitory acitivity, besides exerting a specific effect on hexokinase (glucosone) and UTP-trapping activity.
    Type of Medium: Electronic Resource
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