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  • Freeze-etching  (1)
  • Rat kidney  (1)
  • 1970-1974  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 371-379 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rat ; Electric coupling areas ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed. Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0568
    Keywords: Rat kidney ; Macula densa ; Dark and light cells ; Fixation procedure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Verfasser beschreiben die Ultrastruktur der dunklen Zellen in der Macula densa der Mittelstücke der Ratten-Niere nach Perfusionsfixierung in situ. In Vergleich mit den Resultaten aus einer früheren Versuchsreihe mit Immersionsfixierung ist der prozentuale Anteil der dunklen Zellen etwa viermal geringer, während ihre Ultrastruktur im Prinzip die gleiche geblieben ist. Die Verfasser vertreten die Auffassung, daß die dunklen Macula-Zellen nicht unbedingt Präparationsartefakte sind.
    Notes: Summary The authors describe the ultrastructure of the dark cells in the macula densa of the distal convoluted tubule of the rat kidney after fixation carried out by vascular perfusion in situ. In this case the frequency of the dark cells compared with that of the light cells is about four times lower than in previous investigations using the fixation procedure by immersion, whereas the ultrastructure remains essentially unchanged. However, the authors believe that the dark cells of the macula densa are not necessarily artifacts due to the preparation of the specimens for examination in the electron microscope. At present it is not yet possible to decide if the dark and the light cells are two specific cell types of the macula densa or if they represent two different functional stages of a single cell type.
    Type of Medium: Electronic Resource
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