Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

  • 1
    ISSN: 1573-5001
    Keywords: GATA-1 ; protein-protein interactions ; solution structure ; zinc finger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Zinc fingers (ZnFs) are generally regarded as DNA-binding motifs. However, a number of recent reports have implicated particular ZnFs in the mediation of protein-protein interactions. The N-terminal ZnF of GATA-1 (NF) is one such finger, having been shown to interact with a number of other proteins, including the recently discovered transcriptional co-factor FOG. Here we solve the three-dimensional structure of the NF in solution using multidimensional 1H/15N NMR spectroscopy, and we use 1H/15N spin relaxation measurements to investigate its backbone dynamics. The structure consists of two distorted β-hairpins and a single α-helix, and is similar to that of the C-terminal ZnF of chicken GATA-1. Comparisons of the NF structure with those of other C4-type zinc binding motifs, including hormone receptor and LIM domains, also reveal substantial structural homology. Finally, we use the structure to map the spatial locations of NF residues shown by mutagenesis to be essential for FOG binding, and demonstrate that these residues all lie on a single face of the NF. Notably, this face is well removed from the putative DNA- binding face of the NF, an observation which is suggestive of simultaneous roles for the NF; that is, stabilisation of GATA-1 DNA complexes and recruitment of FOG to GATA-1-controlled promoter regions.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Prostaglandins 2 (1972), S. 441-452 
    ISSN: 0090-6980
    Keywords: [abr] ADP; adenosine diphosphate ; [abr] AMP-PNP; 5'-adenylyl-imidodiphosphate ; [abr] ATP; adenosine triphosphate ; [abr] CTP; cytidine triphosphate ; [abr] GDP; guanosine diphosphate ; [abr] GMP; guanosine monophosphate ; [abr] GTP; guanosine triphosphate ; [abr] PGE"1; prostaglandin E"1 ; [abr] PGE"2; prostaglandin E"2 ; [abr] PY"1; 7-oxa-13-prostynoic acid ; [abr] TSH; thyrotropin ; [abr] UTP; uridine triphosphate ; [abr] cyclic AMP (cAMP); cyclic 3',5'-adenosine monophosphate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...