Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • GENE  (2)
  • IV heparin prophylaxis  (2)
Keywords
Publisher
Years
  • 1
    Keywords: CANCER ; CANCER CELLS ; CELLS ; tumor ; CELL ; COMBINATION ; Germany ; IN-VIVO ; MICROSCOPY ; THERAPY ; VOLUME ; GENE ; gene therapy ; MICE ; ACTIVATION ; prognosis ; colon ; INJECTION ; treatment ; BREAST-CANCER ; PROMOTER ; genetics ; colorectal cancer ; COLORECTAL-CANCER ; chemotherapy ; CANCER-CELLS ; COLON-CANCER ; FLUORESCENCE ; GENE-THERAPY ; GREEN FLUORESCENT PROTEIN ; heredity ; CYP2B1 ; ifosfamide ; ACTIVATING CYTOCHROME-P450 ; cytochrome P450 ; ONCOLOGY ; colon cancer ; QUALITY-OF-LIFE ; ENZYME ; SULFATE ; HISTOLOGY ; COMPOUND ; CANCER-TREATMENT ; animal ; viability ; cytomegalovirus ; FORMULATION ; INTRAPERITONEAL HYPERTHERMIC CHEMOTHERAPY ; ISOPHOSPHORAMIDE MUSTARD ; LAPAROSCOPIC FLUORESCENCE DIAGNOSIS ; peritoneal carcinomatosis ; TARGETED CHEMOTHERAPY
    Abstract: The prognosis of peritoneal spread from gastrointestinal cancer and subsequent malignant ascites is poor, and current medical treatments available are mostly ineffective. Targeted chemotherapy with intraperitoneal prodrug activation may be a beneficial new approach. L293 cells were genetically modified to express the cytochrome P450 enzyme 2B1 under the control of a cytomegalovirus immediate early promoter. This CYP2B1 enzyme converts ifosfamide to its active cytotoxic compounds. The cells are encapsulated in a cellulose sulfate formulation (Capcell(TM)). Adult Balb/c mice were inoculated intraperitoneally with 1 x 10(6) colon 26 cancer cells, previously transfected with GFP to emit a stable green fluorescence, by injection into the left lower abdominal quadrant. Two or five day's later animals were randomly subjected to either i.p. treatment with ifosfamide alone or ifosfamide combined with microencapsulated CYP2B1-expressing cells. Peritoneal tumor volume and tumor viability were assessed 10 days after tumor inoculation by means of fluorescence microscopy, spectroscopy and histology. Early i.p. treatment with ifosfamide and CYP2B1 cells resulted in a complete response. Treatment starting on day 5 and single-drug treatment with ifosfamide resulted in a partial response. These results suggest that targeted i.p. chemotherapy using a combination of a prodrug and its converting enzyme may be a successful treatment strategy for peritoneal spread from colorectal cancer
    Type of Publication: Journal article published
    PubMed ID: 16096652
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: CANCER ; EXPRESSION ; tumor ; carcinoma ; CELL ; Germany ; DISEASE ; DISEASES ; GENE ; GENES ; SAMPLE ; SAMPLES ; cell line ; TISSUE ; LINES ; PATIENT ; SERA ; ANTIGEN ; ANTIGENS ; SKIN ; CELL-LINES ; FREQUENCY ; FIELD ; FREQUENCIES ; RECOGNITION ; tumor antigens ; antibodies ; antibody ; TARGET ; colorectal cancer ; COLORECTAL-CANCER ; CELL-LINE ; LINE ; CANCER-PATIENTS ; CARCINOEMBRYONIC ANTIGEN ; RT-PCR ; IMMUNE-RESPONSE ; IMMUNOTHERAPY ; cancer-germline genes ; MAGE GENES ; MELANOMA PATIENTS ; NY-ESO-1 ; sero-reactivity ; TARGETS ; CANCER PATIENTS ; INTERFERON-GAMMA ; LOCATION ; CYTOLYTIC T-LYMPHOCYTES ; SERUM ; RECOMBINANT ; COLORECTAL-CARCINOMA ; TUMOR-ANTIGENS ; CARCINOMA PATIENTS ; CELL LYMPHOMA ; cTAGE ; GAGE ; MAGE
    Abstract: The expression of 14 individual and two groups of tumor antigens was characterized for colorectal carcinoma by RT-PCR using 26 colorectal carcinoma specimens, eight cell lines, six samples of patients with inflammatory bowl diseases, and nine specimens from different locations of an individual patient with a metastasized rectal carcinoma. The most frequently detected mRNAs were MAGE-A1 (58%), GAGE-3-7 (54%), and cTAGE-5a (31%). At medium frequencies (12-19%) we found cTAGE-1, MAGE-A2, se57-1, RAGE-4, and GAGE-1,2,8, while other tumor antigens were expressed rarely (〈9%). 85% of the samples were positive for at least one of the most frequently expressed antigens. Using a secondary SEREX approach and sera of eight colorectal cancer patients we found reactive antibodies against recombinant cTAGE-l (2 sera), se57-1 (2), truncated GAGE (1), and MAGE-Al (1). We conclude that certain cancer-germline genes can be detected in colorectal cancer and might therefore be promising targets for immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 15142679
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1435-2451
    Keywords: Multiple trauma patients ; IV heparin prophylaxis ; Thrombo-embolic complications
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das Risiko thromboembolischer Komplikationen ist beim Polytrauma infolge verschiedener pathophysiologischer Vorgänge erhöht. Ziel dieser Studie war es den Einfluß einer kontinuierlichen IV Heparin-Prophylaxe (10 000 IE/24h) beim Polytrauma zu untersuchen. Retrospektiv wurden 72 obduzierte Todesfälle von 373 Polytraumen aus den Jahren 1984 und 1985 analysiert. Bei einem Patienten waren multiple periphere Makrolungenembolien für den Tod mitverantwortlich. Fulminante Lungenembolien, Becken- und tiefe Beinvenenthrombosen wurden im Sektionsgut nicht gefunden.
    Notes: Summary The risk of thrombo-embolic complications in multiple injured patients is increased due to several pathophysiological factors. The purpose of this study was to analyse the effect of a continuous IV heparin prophylaxis (10000IE/24h) in polytraumatized patients. A post-mortem evaluation of 72 deceased patients out of 373 multiple trauma patients treated during 1984–1985 was performed. In one patient multiple major pulmonary emboli were found to contribute to his death. No deep vein thrombosis as well as no fulminant pulmonary embolism were found in any case.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1435-2451
    Keywords: Multiple trauma patients ; IV heparin prophylaxis ; Thromboembolic complications ; Polytrauma ; Heparin-Prophylaxe ; Lungenemboliehliufigkeit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das Risiko thromboembolischer Komplikationen ist beim Polytrauma infolge verschiedener pathophysiologischer Vorgänge erhöht. Ziel dieser Studie war es, den Einfluss einer kontinuierlichen IV Heparin Prophylaxe (10 000 IE/24 h) beim Polytrauma zu untersuchen. Retrospektiv wurden 72 obduzierte Todesfälle von 373 Polytraumen aus den Jahren 1984 und 1985 analysiert. Bei einem Patienten waren multiple periphere Makrolungenembolien für den Tod mitverantwortlich. Fulminante Lungenembolien, Becken- und tiefe Beinvenenthrombosen wurden im Sektionsgut nicht gefunden.
    Notes: Summary The risk of thromboembolic complications is increased in patients with multiple injuries patients due to several pathophysiological factors. The purpose of this study was to analyze the effect of continuous IV heparin prophylaxis (10,000 IU/24 h) in patients with polytrauma. A postmortem evaluation was performed based on 72 deceased patients out of 373 multiple trauma patients treated during 1984–1985. In one patient, multiple major pulmonary emboli were found to contribute to his death. No deep vein thrombosis or fulminant pulmonary embolism was found in any of the cases.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...