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  • Gonadotropic cells  (2)
  • AAV2 VECTORS  (1)
  • biventricular pacing
  • 1
    Keywords: PEPTIDE ; CELLS ; EXPRESSION ; CELL ; Germany ; human ; IN-VIVO ; THERAPY ; VIVO ; SAMPLE ; SAMPLES ; INFECTION ; SERA ; DOMAIN ; BINDING ; LIMITATION ; antibodies ; antibody ; NEUTRALIZING ANTIBODIES ; virus ; IDENTIFICATION ; VECTORS ; VECTOR ; EFFICIENT ; TRAFFICKING ; REGION ; REGIONS ; SURFACE ; EPITOPE ; EPITOPES ; PREVALENCE ; adeno-associated virus ; TYPE-2 ; ADENOVIRUS ; AAV ; AAV2 VECTORS ; AFFINITY ; CANINE PARVOVIRUS ; DOMAINS ; EMPTY CAPSIDS ; NEUTRALIZATION ; immunogenic epitopes ; AAV antibodies ; human serum sampl
    Abstract: The high prevalence of human serum antibodies against adeno-associated virus type 2 (AAV) vectors represents a potential limitation for in vivo applications. Consequently, the development of AAV vectors able to escape antibody binding and neutralization is of importance. To identify capsid domains which contain major immunogenic epitopes, six AAV capsid mutants carrying peptide insertions in surface exposed loop regions (I-261, I-381, I-447, I-534, I-573, I-587) were analyzed. Two of these mutants, I-534 and I-573, showed an up to 70% reduced affinity for AAV antibodies as compared to wild-type AAV in the majority of serum samples. In addition, AAV mutant I-587 but not wild-type AAV efficiently transduced cells despite the presence of neutralizing antisera. Taken together, the results show that major neutralizing effects of human AAV antisera might be overcome by the use of AAV capsid mutants
    Type of Publication: Journal article published
    PubMed ID: 14625569
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  • 2
    ISSN: 1432-0878
    Keywords: Pars tuberalis ; Immunofluorescence ; Gonadotropic cells ; βLH, βFSH ; Monkey, Macaca irus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La pars tuberalis de l'hypophyse du Singe Macacus irus entoure la tige infundibulaire jusqu'à l'éminence médiane. En techniques histologiques, elle apparaît constituée de plusieurs assises cellulaires d'aspect “chromophobe”. On y observe quelques cellules PAS-positives réagissant simultanément avec le bleu Alcian (pH3.0). En technique d'immunofluorescence indirecte, des cellules dispersées sont mises en évidence uniquement avec un anticorps anti-βoLH; ces cellules ne réagissent pas avec un anticorps anti-βhFSH. L'utilisation d'anticorps anti-hGH, anti-hPRL, anti-ACTH, anti-MSH, anti-LPH et antiendorphines ne permet pas de révéler des cellules immunoréactives. Quelques cellules réagissant avec un anticorps anti-βhTSH s'observent à la base de la tige hypophysaire (zona tuberalis), c'est-à-dire au-delà de la pars tuberalis proprement dite. Ces résultats sont confrontés à ceux rapportés dans la littérature.
    Notes: Summary The pars tuberalis of the hypophysis in the monkey Macaca irus encompasses the hypophysial stem up to the median eminence. Histologically, it consists of several layers of chromophobic cells. A few PAS1-positive cells also stainable with Alcian blue (pH 3.0) can be observed among the unstained elements. Using the indirect immunofluorescence antibody technique, scattered immunoreactive cells were revealed with the anti-βoLH antibody; these cells did not react with the anti-βhFSH antibody. In contrast, the immunoreactions to anti-hGH, anti-hPRL, anti-ACTH, anti-MSH, anti-LPH and anti-endorphin sera were completely negative. Single cells reacting with the anti-βhTSH serum were observed at the inferior end of the hypophysial stalk (zona tuberalis), i.e., beyond the pars tuberalis proper. These results are compared with data reported in the literature.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-8595
    Keywords: biatrial pacing ; biventricular pacing ; pacemaker indication ; coronary sinus electrodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The artificial activation of the heart modifies the mechanics of contraction and relaxation. While only little basic research has been addressed to this question, clinical observations showed that for hypertrophic as well as dilated cardiomyopathies appropriate pacing techniques can be useful. Pacing can influence the activation sequence. The spread out from a single site is slow, and so hypercontractility deminshed. With the use of multiple electrodes, two atrial and/or two ventricular, conduction delays in the atria or ventricles can be eliminated. Synchronisation of the cardiac activation has an anti-arrhythmic and positiv inotropic effect. This may lead to new indications for pacemakers or better to be named cardiac synchronisers.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Monkeys ; Vervet (Cercopithecus aethiops) ; Baboon (Papio hamadryas) ; Gonadotropic cells ; Pars distalis ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Des anticorps anti-LH ovine, anti-βLH ovine et anti-βFSH humaine ont été utilisés pour localiser les cellules gonadotropes dans la pars distalis de l'hypophyse des Singes Cercopithecus aethiops et Papio hamadryas. Trois catégories cellulaires distinctes, réagissant avec des anticorps anti-hormones gonadotropes, ont été observées. 85% des cellules immunoréactives identifiées en tant que cellules gonadotropes réagissent simultanément avec les différents anticorps mentionnés; 10% des cellules gonadotropes réagissent seulement avec l'anticorps anti-βoLH et 5% de ces cellules seulement avec l'anticorps anti-βhFSH. La comparaison avec des coupes adjacentes traitées par divers anticorps autres que les anticorps anti-gonadotropines prouve que les “cellules gonadotropes” de ces Singes ne réagissent jamais simultanément avec l'un ou l'autre de ces anticorps.
    Notes: Summary Antisera against oLH1, βoLH and βhFSH were used to localize gonadotropic cells in the pars distalis of Cercopithecus aethiops and Papio hamadryas. Three separate cell types were observed for FSH and LH: 85% of immunohistochemically identified gonadotropic cells reacted to all the various antisera; 10% reacted with the anti-βLH antibody only; and 5% with the anti-βhFSH antibody only. Comparisons between adjacent serial sections treated with various antisera, other than anti-gonadotropic hormones, demonstrated that the “gonadotropic cells” of these monkeys did not respond to these antisera.
    Type of Medium: Electronic Resource
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