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  • 1
    Keywords: IN-VITRO ; NITRIC-OXIDE SYNTHASE ; BONE-MARROW ; HEMATOPOIETIC STEM-CELLS ; COLONY-STIMULATING FACTOR ; RHEUMATOID-ARTHRITIS ; CORONARY-ARTERY-DISEASE ; ACUTE MYOCARDIAL-INFARCTION ; ACUTE ISCHEMIC-STROKE ; LEFT-VENTRICULAR FUNCTION
    Abstract: The term endothelial progenitor cell (EPC) was established more than 10 years ago and is used to refer to a group of circulating cells that display endothelial lineage qualities and are able to home to areas of ischemia or vascular injury and to facilitate the repair of damaged blood vessels or develop new vessels as needed. This chapter reviews the current lineage relationships among all the cells called EPC and will clear the terminology used in EPC research. Furthermore, an overview of the clinical and in vitro research, as well as cytokine and drug interactions and potential EPC applications, is given.
    Type of Publication: Journal article published
    PubMed ID: 24016523
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  • 2
    ISSN: 1432-2048
    Keywords: Abscisic acid (desiccation tolerance) ; Callus (desiccation tolerance) ; Craterostigma ; Desiccation tolerance (protein, RNA induction) ; Resurrection plant RNA induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leaves of the resurrection plant Craterostigma plantagineum Hochst, can be desiccated up to 1% relative water content and are still viable after rehydration. To clone genes related to this extreme desiccation tolerance, an in-vitro system was first developed which allows the induction of the same resurrection response in callus tissue upon treatment with abscisic acid (ABA). Several proteins and in-vitro-synthesized polypeptides were then identified which can be induced both in desiccation-tolerant, naturally dried leaves and in ABA-treated calli surviving after rehydration. Complementary-DNA clones corresponding to mRNAs expressed only in desiccation-tolerant tissues were obtained and classified into several gene families. In hybrid-selected translation experiments, representative cDNA clones were associated with water stress and ABA-inducible polypeptides abundantly expressed in dried leaves and ABA-treated calli. The expression pattern of several of these abundant transcripts was analyzed in RNA-hybridization experiments. Upon stress or ABA treatment the transcription levels increased rapidly, but they declined after relief from the stress state. This, together with data on genomic copy numbers indicated that a set of abundantly expressed genes are involved in the desiccation process of resurrection plants. Data on endogenous ABA contents before and after stress applications and on the physiological effects of exogenous ABA treatments indicate that in Craterostigma plantagineum the induction of an extreme desiccation tolerance is mediated by this plant hormone.
    Type of Medium: Electronic Resource
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