Keywords:
CELLS
;
tumor
;
CELL
;
human
;
COMMON
;
DISEASE
;
SITES
;
PROTEINS
;
SAMPLE
;
SAMPLES
;
TUMORS
;
TIME
;
PATIENT
;
DNA
;
SKIN
;
papillomavirus
;
antibody
;
IN-SITU
;
LESIONS
;
COPY NUMBER
;
human papillomavirus
;
GENOTYPES
;
HPV
;
REPLICATION
;
glutathione-S-transferase
;
PSORIASIS
;
EPIDERMODYSPLASIA-VERRUCIFORMIS
;
hair
;
GENOTYPE
;
NONMELANOMA SKIN-CANCER
;
USA
;
PLUCKED EYEBROW HAIRS
;
CLINICAL-ASPECTS
;
HAIRS
;
HUMAN-PAPILLOMAVIRUS-DNA
Abstract:
Epidermodysplasia verruciformis (EV) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to beta-genus human papillomavirus (HPV). Earlier studies reported high copy numbers of HPV-DNA in nearly all skin tumors from EV patients, but neither HPV replication status in non-lesional skin nor anti-HPV seroreactivity in these patients have been reported yet. We therefore performed a comprehensive viral load analysis for the more common beta-HPV types on skin samples and plucked eyebrow hairs from four EV patients treated at our dermatology department. The results clearly demonstrate that they carry a multiplicity (up to eighteen types) of beta-HPV genotypes in both skin sites. Worthy of note, a high intrapatient concordance for specific types between hair bulbs and skin biopsies was observed and the same beta-PV profile was maintained over time. Viral load analysis revealed a load range between less than one HPV-DNA copy per 100 cells to more than 400 HPV-DNA copies per cell in both eyebrow hairs and skin proliferative lesions. Evaluation of seroreactivity to beta-HPV types in the four EV patients revealed that antibodies against the 16 beta-HPV were significantly more prevalent and showed higher titers than in the controls
Type of Publication:
Journal article published
Deep Link:
http://www.dkfz.de/cgi-bin/sel?http://www.dkfz.de/PublicationManager/Show/ShowJournal.aspx%3fpublishedId=7604
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