Key words Arabidopsis HSP18.2
GUS chimeric gene
Springer Online Journal Archives 1860-2000
Abstract The expression of the Arabidopsis heat-shock protein (HSP) 18.2 promoter β-d-glucuronidase (GUS) chimera gene was studied in various organs of the transgenic Nicotiana plumbaginifolia during the recovery phase at normal temperatures (20–22 °C) following heat-shock (HS) treatment. The optimum HS temperature for GUS activity in the anthers, petals and capsules was 42 °C, but in immature seeds and the placentas of capsules it was 36 °C and 39 °C, respectively. No apparent GUS activity was observed in any organs except for dry seeds after HS at 45 °C, although the activity in dry seeds was apparent even after HS at 48 °C. After HS at 42 °C, GUS activity in the flower tissues was the highest before anthesis and declined thereafter.
Type of Medium: