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  • CD40L  (2)
  • Hemidesmosome  (2)
  • Th. junceum  (2)
  • Springer  (6)
  • International Union of Crystallography (IUCr)
Collection
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  • Springer  (6)
  • International Union of Crystallography (IUCr)
Years
  • 1
    ISSN: 1569-8041
    Keywords: CD30L ; CD40L ; FasL ; Hodgkin's disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Reed–Sternberg cells of Hodgkin's disease express CD30and CD40 receptors. The ligands for these receptors have been reported to have pleiotropic biologic activities in vitro, including induction of cell death. Expression of the ligands for these receptors in lymph nodes involved with Hodgkin's disease is not known. Purpose: The purpose of this study was to examine CD30 ligand (L) andCD40L expression in lymph nodes of patients with Hodgkin's disease, and to study CD30L expression on nodal lymphocyte subsets. Materials and methods: CD30L expression on subsets of lymphocytes of five lymph nodes involved with Hodgkin's disease was determined by two-color FACScan. Messenger RNA expression of CD30L and CD40L was determined by there verse-transcriptase polymerase chain reaction (RT-PCR) method performed on seven specimens involved with Hodgkin's disease (five lymph nodes and two spleens). Results: Four of seven specimens (57%) contained cells that expressed CD30L mRNA and three specimens (43%) containedCD40L-expressing cells. The mean percentage of nodal lymphocytes expressingCD30L surface protein was ≤20%. Conclusion: Hodgkin's disease lymph nodes and spleens frequently lackCD30L- and CD40L-expressing cells, and when CD30L is expressed, it is usually detected on few numbers of lymphocytes. The differences in the level of expression of these ligands in Hodgkin's disease lymph nodes may be related to the disease's clinical behavior.
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  • 2
    ISSN: 1569-8041
    Keywords: CD30L ; CD40L ; FasL ; Hodgkin's disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Reed–Sternberg cells of Hodgkin's disease express CD30and CD40 receptors. The ligands for these receptors have been reported tohave pleiotropic biologic activities in vitro, including induction of celldeath. Expression of the ligands for these receptors in lymph nodes involvedwith Hodgkin's disease is not known. Purpose: The purpose of this study was to examine CD30 ligand (L) andCD40L expression in lymph nodes of patients with Hodgkin's disease, and tostudy CD30L expression on nodal lymphocyte subsets. Materials and methods: CD30L expression on subsets of lymphocytes of fivelymph nodes involved with Hodgkin's disease was determined by two-colorFACScan. Messenger RNA expression of CD30L and CD40L was determined by thereverse-transcriptase polymerase chain reaction (RT-PCR) method performed onseven specimens involved with Hodgkin's disease (five lymph nodes and twospleens). Results: Four of seven specimens (57%) contained cells thatexpressed CD30L mRNA and three specimens (43%) containedCD40L-expressing cells. The mean percentage of nodal lymphocytes expressingCD30L surface protein was ≤20%. Conclusion: Hodgkin's disease lymph nodes and spleens frequently lackCD30L- and CD40L-expressing cells, and when CD30L is expressed, it is usuallydetected on few numbers of lymphocytes. The differences in the level ofexpression of these ligands in Hodgkin's disease lymph nodes may be relatedto the disease's clinical behavior.
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  • 3
    ISSN: 1432-2242
    Keywords: Thinopyrum ponticum ; Th. intermedium ; Th. junceum ; Wheat ; BYDV ; Genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-five partial amphiploids (2n=8x=56), which were derived from hybrids of wheat (Triticum aestivum L.) with either Thinopyrum ponticum (Podpera) Liu & Wang, Th. intermedium (Host) Barkworth & D. Dewey, or Th. junceum (L.) A. Löve, were assayed for resistance to BYDV serotype PAV by slot-blot hybridization with viral cDNA of a partial coat protein gene. Three immune lines were found among seven partial amphiploids involving Th. ponticum. Seven highly resistant lines were found in ten partial amphiploids involving Th. intermedium. None of eight partial amphiploids or 13 addition lines of Chinese Spring — Th. junceum were resistant to BYDV. Genomic in situ hybridization demonstrated that all of the resistant partial amphiploids, except TAF46, carried an alien genome most closely related to St, whether it was derived from Th. ponticum or Th. intermedium. The two partial amphiploids carrying an intact E genome of Th. ponticum are very susceptible to BYDV-PAV. In TAF46, which contains three pairs of St- and four pairs of E-genome chromo somes, the gene for BYDV resistance has been located to a modified 7 St chromosome in the addition line L1. This indicates that BYDV resistance in perennial polyploid parents, i.e., Th. ponticum and Th. intermedium, of these partial amphiploids is probably controlled by a gene(s) located on the St-genome chromosome(s).
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  • 4
    ISSN: 1432-2242
    Keywords: Key wordsThinopyrum ponticum ; Th. intermedium ; Th. junceum ; Wheat ; BYDV ; Genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-five partial amphiploids (2n=8x=56), which were derived from hybrids of wheat (Triticum aestivum L.) with either Thinopyrum ponticum (Podpera) Liu & Wang, Th. intermedium (Host) Barkworth & D. Dewey, or Th. junceum (L.) A. Löve, were assayed for resistance to BYDV serotype PAV by slot-blot hybridization with viral cDNA of a partial coat protein gene. Three immune lines were found among seven partial amphiploids involving Th. ponticum. Seven highly resistant lines were found in ten partial amphiploids involving Th. intermedium. None of eight partial amphiploids or 13 addition lines of Chinese Spring –Th. junceum were resistant to BYDV. Genomic in situ hybridization demonstrated that all of the resistant partial amphiploids, except TAF46, carried an alien genome most closely related to St, whether it was derived from Th. ponticum or Th. intermedium. The two partial amphiploids carrying an intact E genome of Th. ponticum are very susceptible to BYDV-PAV. In TAF46, which contains three pairs of St- and four pairs of E-genome chromosomes, the gene for BYDV resistance has been located to a modified 7St chromosome in the addition line L1. This indicates that BYDV resistance in perennial polyploid parents, i.e., Th. ponticum and Th. intermedium, of these partial amphiploids is probably controlled by a gene(s) located on the St-genome chromosome(s).
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  • 5
    ISSN: 1434-0879
    Keywords: Basement membrane ; Tumor invasion ; Transitional cell carcinoma ; Degradation ; Neosynthesis ; Hemidesmosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study describes ultrastructural alterations in the basement membrane (BM) of rat bladder with invasive transitional cell carcinoma (TCC) induced by N-butyl-N-(4-hydroxybutyl)nitrosamine. Various alterations including thickening, degradation and neosynthesis were found in the bladder BM of one rat with invasive TCC. Focal destruction of both the BM lamina zones was found in addition to partially degraded BMs showing focal degradation and loss of only the BM lamina rara. Neosynthesis of complete BM including the lamina rara and lamina censa was observed surrounding the nests of carcinoma cells deep in the stroma, while neosynthesis of incomplete BM including only a lamina densa-like structure was also found around carcinoma cells which had just crossed the BM into the adjacent stroma from the original tumor masses. There was an increased hemidesmosomal frequency in some areas of thickened BM, and focal loss of hemidesmosome in the areas of degraded BM. It is suggested that BM degradation may take place in two steps, and that BM neosynthesis may also be a two-step process in invasive TCC of rat bladder.
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  • 6
    ISSN: 1432-069X
    Keywords: 1-2B7B monoclonal antibody ; Hemidesmosome ; Epidermal-dermal junction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunohistochemical and immunochemical analyses were performed on a monoclonal antibody designated 1-2B7B which was derived from immunizing mice with human prostate epithelial tissue. The 1-2B7B antigen was expressed not only along the acinous basement membrane zone (BMZ) of the prostate and testis, but also along the BMZ of the epithelia of several other organs including the skin, oesophagus, urinary bladder, ureter, stomach, intestine and bile duct. The antigenic epitope was not expressed in these tissues of lower mammals. Immunoelectron microscopic studies on normal human skin revealed that the 1-2B7B antigen was localized mainly just beneath the hemidesmosomes of basal keratinocytes, but not beneath melanocytes. Indirect immunofluorescence and immunoelectron microscopic studies on 1 M NaCl-split skin confirmed that this antigen was not separated from the cytoplasmic membrane of basal cells after salt treatment. SDS polyacrylamide gel electrophoresis of immunochemically purified protein from the epidermis demonstrated the molecular weight of the antigen to be 120 kDa. 1-2B7B monoclonal antibody should be a useful probe for studying the pathomechanism of some blistering diseases, as well as the assembly and function of the epidermal-dermal junction.
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