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  • INTERCELLULAR-ADHESION MOLECULE-1  (1)
  • 1
    Keywords: RECEPTOR ; CELLS ; CELL ; Germany ; human ; MICROSCOPY ; NEW-YORK ; HYBRIDIZATION ; PROTEIN ; PROTEINS ; ACTIVATION ; kidney ; IN-SITU ; immunohistochemistry ; UP-REGULATION ; COMPONENT ; PCR ; EPITHELIAL-CELLS ; POLYMERASE-CHAIN-REACTION ; immune response ; IMMUNE-RESPONSE ; CHAIN-REACTION ; REJECTION ; SEGMENTS ; LOCATION ; TNF-ALPHA ; in situ hybridization ; CYTOKINE ; CHAIN ; secretion ; polymerase chain reaction ; BLOOD MONONUCLEAR-CELLS ; INTERCELLULAR-ADHESION MOLECULE-1 ; USA ; immunology ; distal tubule ; ACUTE TUBULAR-NECROSIS ; collecting ducts ; CONNECTING TUBULE ; CYTOKINE RESPONSES ; GAMMA-INDUCING FACTOR ; INTERLEUKIN-18
    Abstract: We determined the cellular location of interleukin-18 (IL-18) and caspase-1 and the purinergic receptor P2X7, two proteins necessary for its activation and secretion. The mRNA and protein of IL-18 were detectable in normal human kidney by means of polymerase chain reaction (PCR), in situ hybridization, and Western blot. Immunohistochemistry located IL-18 to nephron segments containing calbinbin-D28k or aquaporin-2 that suggest location in the distal convoluted and the connecting tubule and to parts of the collecting duct. IL-18 was not detected in the thick ascending limb of Henle. Confocal microscopy showed that IL-18 was expressed in cells negative for calbindin-D28k and for aquaporin-2 but positive for the vacuolar H+ -ATPase. This demonstrates that the intercalated cells produce IL-18. These segments were also positive for caspase-1 and P2X7 that are essential for IL-18 secretion. Our results show that IL-18 is constitutively expressed by intercalated cells of the late distal convoluted tubule, the connecting tubule, and the collecting duct of the healthy human kidney. Since IL-18 is an early component of the inflammatory cytokine cascade, its location suggests that renal intercalated cells may contribute to immediate immune response of the kidney
    Type of Publication: Journal article published
    PubMed ID: 17687255
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