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  • Immunofluorescence  (5)
  • Dysgnathiechirurgie
  • biventricular pacing
  • 1
    ISSN: 1432-0878
    Keywords: Hypophysis, pars distalis ; Somatotropic and prolactin cells ; Immunofluorescence ; Monkey, Macacus irus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using antibodies against human growth hormone and ovine prolactine, it is possible to identify somatotropic and prolactin cells in the anterior lobe of the hypophysis (pars distalis) of the monkey, Macacus irus. These cells are present in both adult and infant male and female monkeys. The density of the somatotropic cells is greater at the periphery of the anterior lobe. Prolactin cells are of two types: one type is dispersed throughout the pars distalis and consists of small and large cells, the other type forms clusters of large cells.
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  • 2
    ISSN: 1432-0878
    Keywords: Monkeys ; Vervet (Cercopithecus aethiops) ; Baboon (Papio hamadryas) ; Gonadotropic cells ; Pars distalis ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Des anticorps anti-LH ovine, anti-βLH ovine et anti-βFSH humaine ont été utilisés pour localiser les cellules gonadotropes dans la pars distalis de l'hypophyse des Singes Cercopithecus aethiops et Papio hamadryas. Trois catégories cellulaires distinctes, réagissant avec des anticorps anti-hormones gonadotropes, ont été observées. 85% des cellules immunoréactives identifiées en tant que cellules gonadotropes réagissent simultanément avec les différents anticorps mentionnés; 10% des cellules gonadotropes réagissent seulement avec l'anticorps anti-βoLH et 5% de ces cellules seulement avec l'anticorps anti-βhFSH. La comparaison avec des coupes adjacentes traitées par divers anticorps autres que les anticorps anti-gonadotropines prouve que les “cellules gonadotropes” de ces Singes ne réagissent jamais simultanément avec l'un ou l'autre de ces anticorps.
    Notes: Summary Antisera against oLH1, βoLH and βhFSH were used to localize gonadotropic cells in the pars distalis of Cercopithecus aethiops and Papio hamadryas. Three separate cell types were observed for FSH and LH: 85% of immunohistochemically identified gonadotropic cells reacted to all the various antisera; 10% reacted with the anti-βLH antibody only; and 5% with the anti-βhFSH antibody only. Comparisons between adjacent serial sections treated with various antisera, other than anti-gonadotropic hormones, demonstrated that the “gonadotropic cells” of these monkeys did not respond to these antisera.
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  • 3
    ISSN: 1432-0878
    Keywords: Pars tuberalis ; Immunofluorescence ; Gonadotropic cells ; βLH, βFSH ; Monkey, Macaca irus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La pars tuberalis de l'hypophyse du Singe Macacus irus entoure la tige infundibulaire jusqu'à l'éminence médiane. En techniques histologiques, elle apparaît constituée de plusieurs assises cellulaires d'aspect “chromophobe”. On y observe quelques cellules PAS-positives réagissant simultanément avec le bleu Alcian (pH3.0). En technique d'immunofluorescence indirecte, des cellules dispersées sont mises en évidence uniquement avec un anticorps anti-βoLH; ces cellules ne réagissent pas avec un anticorps anti-βhFSH. L'utilisation d'anticorps anti-hGH, anti-hPRL, anti-ACTH, anti-MSH, anti-LPH et antiendorphines ne permet pas de révéler des cellules immunoréactives. Quelques cellules réagissant avec un anticorps anti-βhTSH s'observent à la base de la tige hypophysaire (zona tuberalis), c'est-à-dire au-delà de la pars tuberalis proprement dite. Ces résultats sont confrontés à ceux rapportés dans la littérature.
    Notes: Summary The pars tuberalis of the hypophysis in the monkey Macaca irus encompasses the hypophysial stem up to the median eminence. Histologically, it consists of several layers of chromophobic cells. A few PAS1-positive cells also stainable with Alcian blue (pH 3.0) can be observed among the unstained elements. Using the indirect immunofluorescence antibody technique, scattered immunoreactive cells were revealed with the anti-βoLH antibody; these cells did not react with the anti-βhFSH antibody. In contrast, the immunoreactions to anti-hGH, anti-hPRL, anti-ACTH, anti-MSH, anti-LPH and anti-endorphin sera were completely negative. Single cells reacting with the anti-βhTSH serum were observed at the inferior end of the hypophysial stalk (zona tuberalis), i.e., beyond the pars tuberalis proper. These results are compared with data reported in the literature.
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  • 4
    ISSN: 1572-8595
    Keywords: biatrial pacing ; biventricular pacing ; pacemaker indication ; coronary sinus electrodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The artificial activation of the heart modifies the mechanics of contraction and relaxation. While only little basic research has been addressed to this question, clinical observations showed that for hypertrophic as well as dilated cardiomyopathies appropriate pacing techniques can be useful. Pacing can influence the activation sequence. The spread out from a single site is slow, and so hypercontractility deminshed. With the use of multiple electrodes, two atrial and/or two ventricular, conduction delays in the atria or ventricles can be eliminated. Synchronisation of the cardiac activation has an anti-arrhythmic and positiv inotropic effect. This may lead to new indications for pacemakers or better to be named cardiac synchronisers.
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  • 5
    ISSN: 1432-2307
    Keywords: Human pituitary adenoma ; Acromegaly ; Light and electron microscopy ; Immunofluorescence ; Radioimmunoassay of GH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 22 pure GH cell adenomas and 9 mixed GH cell-prolactin cell adenomas with acromegaly, we compare the morphological and functional data (secretory activity and granular appearance) with GH levels (radioimmunoassays) in the blood and in the tumor. According to morphological criteria, the secretory activity is marked in 13 cases (Group I), mild in 9 cases (Group II), and weak in 9 others (Group III). The mean values of the plasma GH levels in the 3 groups (80 ng/ml±22; 26.5 ng/ml±2; and 16.89 ng/ml±2 respectively) are significantly different. In 17 densely granulated adenomas and 14 sparsely granulated adenomas, the plasma GH values were very variable. The mean levels of these 2 groups (49.76 ng/ml±22 and 41.8 ng/ml±7.8 respectively) are not significantly different. The GH concentrations in the tumor were also very variable (358 to 78,900 ng/mg). Their highly significant relationship with the granular appearance is an indirect proof of the granular localisation of GH. We distinguish between 4 functional aspects of the GH cell adenoma which define the different levels of synthesis, storage, and excretion. The secretory activity of the GH adenomatous cell varies with the adenomas and differs from that of the normal cell.
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  • 6
    ISSN: 1432-0878
    Keywords: Wulzen's cone ; Immunofluorescence ; Bovine adenohypophysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le cône de Wulzen de l'adénohypophyse bovine présente un développement et une disposition générale variables. Il est accolé à la pars intermedia dont il n'est séparé par aucune cloison conjonctive. Du côté de la fente hypophysaire, il est revêtu par un épithélium simple, cubique. En immunofluorescence, on observe la présence de divers types de cellules glandulaires: les plus abondantes sont des cellules mises en évidence par un anticorps anti-oPRL, isolées ou groupées en amas. D'autres cellules réagissent avec des anticorps anti-hGH, et anti-bLH, anti-βoLH ou anti-/mTSH. Quelques cellules réagissent simultanément avec des anticorps anti-βMSH, anti-1–24ACTH, anti-17–39ACTH, anti-βLPH et anti-βendorphine. Mises à part les nombreuses cellules à prolactine, les autres types cellulaires apparaissent constamment sous l'aspect d'éléments isolés. Nous n'avons pas observé de cellules réagissant avec des anticorps anti-leu-enképhaline, antiinet-enképhaline ou anti-calcitonine ni dans le cône de Wulzen, ni dans la pars distalis et dans la pars intermedia.
    Notes: Summary The Wulzen's cone of the bovine adenohypophysis presents a variable development and general arrangement. It is joined to the pars intermedia with no intervening connective tissue. It is covered by a single layer of cubical cell epithelium on the side of the hypophysial cleft. Immunofluorescence reveals the presence of different glandular cell types. The most abundant cells are those demonstrated by an anti-oPRL antibody and are either isolated or clustered. Other cells react with anti-hGH, anti-bLH, anti-βoLH or anti-βhTSH antibodies. Some cells react simultaneously with anti-βMSH, anti-1–24ACTH, anti-17–39ACTH, anti-βLPH and anti-βendorphin antibodies. Cell types other than the numerous prolactin cells appear only as isolated elements. We did not observe cells reacting with anti-leu-enkephalin, anti-met-enkephalin or anti-calcitonin antibodies either in the Wulzen's cone or in the pars distalis or pars intermedia.
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  • 7
    ISSN: 1434-3940
    Keywords: Schlüsselwörter ; Dysgnathiechirurgie ; Operationssimulation ; Weichgewebesimulation ; Computersimulation ; Keywords ; Orthognathic surgery ; Preoperative planning ; Soft tissue simulation ; Computer-aided simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Background In addition to standard X-rays, photographic documentation, cephalometric and model analysis, a computer-aided, three-dimensional (3D) simulation system has been developed in close cooperation with the Institute of Communications of the Friedrich-Alexander-Universität Erlangen-Nürnberg. With this simulation system a photorealistic prediction of the expected soft tissue changes can be made. Prerequisites are a 3D reconstruction of the facial skeleton and a 3D laser scan of the face. After data reduction, the two data sets can be matched. Cutting planes enable the transposition of bony segments. The laser scan of the facial surface is combined with the underlying bone via a five-layered soft tissue model to convert bone movements on the soft tissue cover realistically. Conclusion Further research is necessary to replace the virtual subcutaneous soft tissue model by correct, topographic tissue anatomy.
    Notes: Hintergrund Im Rahmen eines Sonderforschungsbereichs der Deutschen Forschungsgemeinschaft (SFB 603) wurde in Zusammenarbeit mit dem Lehrstuhl für Nachrichtentechnik der Universität Erlangen-Nürnberg ein computergestütztes Simulationssystem zur dreidimensionalen, fotorealistischen Vorhersage von Weichgewebeveränderungen nach orthognathen Eingriffen entwickelt. Voraussetzung sind 3D-CT-Datensätze des Gesichtsschädels sowie eine ebenfalls dreidimensionale Laserabtastung der Gesichtsoberfläche. Beide Datensätze können nach Datenreduktion über ein mathematisches Verfahren so miteinander verknüpft werden, dass mit Hilfe so genannter “cutting planes” Verlagerungen von Knochensegmenten auf das bedeckende Weichgewebe realitätsnah übertragen werden können. Schlussfolgerung Es bedarf weiterer Forschungsanstrengungen, um auch die subkutanen Weichgewebelagen so in das Simulationsmodell zu integrieren, dass noch bestehende Abweichungen korrigiert werden können.
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