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  • Dextran-modified poly(methyl methacrylate) latex particles  (1)
  • Key words: Interleukin 13 — Polymorphonuclear neutrophil — Prostaglandin E2— Cyclooxygenase 2 — Neutral esterase  (1)
  • (Acremonian)
  • Springer  (2)
  • 1995-1999  (2)
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Publisher
  • Springer  (2)
Years
  • 1995-1999  (2)
Year
  • 1
    ISSN: 1435-1536
    Keywords: Key words Isothermal equilibrium adsorption ; Concanavalin A ; Dextran-modified poly(methyl methacrylate) latex particles ; Electrostatic/affinity interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Isothermal equilibrium adsorption experiments were carried out to study the adsorption of concanavalin A (Con A) on dextran-modified poly(methyl methacrylate) (PMMA) latex particles. Three PMMA particles with various levels of dextran modification were selected for study: 0% (designated as D0), 1.24% (D20), and 2.45% (D75) based on total polymer weight. The Langmuir model is applicable to both D0 and D20 systems, although the data for the D20 system are somewhat scattered. On the other hand, the amount of Con A adsorbed per gram polymer particles (q*) versus the Con A concentration in water (c*) curve for the D75 system cannot be described by the Langmuir model. The deviation is attributed to the formation of a crosslinked network structure, caused by specific binding of the dimeric Con A molecules onto two neighboring particles with grafted dextran. The ratio of the initial number of Con A molecules to the initial number of active binding sites on the dextran-modified particle surface plays an important role in determining the structure of flocs formed. The maximum amount of Con A adsorbed on the particle surface (q max) is of the order of 10−1 μmol per gram particles and q max in decreasing order is D75 〉 D20 〉 D0. The dissociation constant of the Con A-D20 (or Con A-D75) pair is of the order of 10−1 μmol dm−3 which is 1 order of magnitude smaller than that of the Con A-D0 pair. Thus, the electrostatic interaction between Con A and D0 is much weaker than the affinity interaction between Con A and D20 (or D75). An empirical model is proposed to qualitatively explain the q* versus c* data.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1420-908X
    Keywords: Key words: Interleukin 13 — Polymorphonuclear neutrophil — Prostaglandin E2— Cyclooxygenase 2 — Neutral esterase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: To investigate whether interleukin-13 (IL-13) can affect arachidonic acid metabolism and phagocytic activity of normal human polymorphonuclear neutrophils (PMN).¶Methods: Normal human PMN (1 × 106 cells/ml) were incubated with different concentrations of IL-13 (0.1–10 ng/ml) for a variety of times (30–120 min). Phagocytosis and intracellular cyclooxygenase-2 (COX-2) were detected by flow cytometry. The expression of COX-1 and COX-2 mRNA was detected by RT-PCR. The concentration of PGE2 in the PMN cultured supernatants was determined by EIA.¶Results: We found that IL-13 at an optimal concentration of 1 ng/ml significantly enhanced COX-2 gene expression and PGE2 production (121.57 ± 22.17 pg/ml in IL-13 stimulation vs. 73.16 ± 11.72 pg/ml in controls) by PMN. In addition, IL-13 stimulated PMN phagocytosis via increased complement receptor type 1 (CR1) and type 3 (CR3), but not IgG Fcγ receptor type 3 (FcγRIII). The cytoplasmic neutral esterase activity of PMN was also enhanced by IL-13 stimulation for 24 h.¶Conclusions: These results suggest that IL-13 can stimulate PMN and modulates the inflammatory reactions via the cyclooxygenase pathway.
    Type of Medium: Electronic Resource
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