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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 1 (1978), S. 59-63 
    ISSN: 0148-7280
    Keywords: oocyte maturation ; LH ; ovum culture ; germinal vesicle breakdown ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The purpose of this study was to analyze the effect of luteinizing hormone (LH) on the earliest stage of oocyte maturation - the stage of breakdown of the dictyate nucleus. Oocytes were isolated from the preovulatory follicles of adult, cyclic rats. They were incubated in culture medium with or without 10 μg/ml LH. The cultures were observed continuously for up to 3 hours. Analysis of the rate of disappearance of the germinal vesicle nucleolus revealed that LH accelerated the breakdown process. The median times of disappearance were 91.3 minutes without LH and 62.3 minutes with LH. This is in accord with earlier reports on enhancement of fertilizability of oocytes matured in vitro with LH. Thus, although oocytes mature spontaneously in culture, the maturation remains LH sensitive.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Key words Glyburide ; oral hypoglycaemic agents ; pregnancy.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Non-insulin-dependent diabetes mellitus (NIDDM) is normally treated by oral hypoglycaemic agents, but their use is excluded during pregnancy because of their potential teratogenic and hypoglycaemic effects on the fetus. This caveat was recently questioned as glyburide was shown to cross an isolated cotyledon in vitro in insignificant amounts. In the present study, placental transport of glyburide in vivo was examined as an indispensable step towards clinical trials. Tritiated glyburide, C14 albumin or C14-labelled diazepam were injected into 13, 9 and 11 pregnant rats, respectively and the radioactivity was measured thereafter in maternal blood and in whole fetal extracts. The ratios between radioactivity in fetal tissue to that in maternal blood for glyburide (0.535 ± 0.068) were similar to those of diazepam (0.641 ± 0.057) which readily crosses the placenta. However, they differed significantly from those for albumin (0.048 ± 0.0004) which does not cross. Moreover, glyburide in fetal tissue consistently reflected its concentration in maternal blood when measured at consecutive intervals after intravenous injection in the mother. In contrast, albumin in fetal tissue was low at all time points regardless of its levels in maternal blood when measured at different times after injection. These data suggest that glyburide crosses the placenta of pregnant rats and should therefore be considered with caution as a hypoglycaemic agent in the treatment of gestational diabetes. [Diabetologia (1995) 38: 753–756]
    Type of Medium: Electronic Resource
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