Life Sciences (general)
Wiley InterScience Backfile Collection 1832-2000
Process Engineering, Biotechnology, Nutrition Technology
To understand and control the dynamics of microbial growth and metabolism, a theoretical background based on segregated models is necessary, and therefore flow cytometry is the suitable measuring method.It is shown that additional information to the usual mean-value information, and to the well-known cytometric methods for determining DNA content and size/shape measurement by scattered light, like the distribution of membrane sterol content of baker's yeast, leads to a more detailed knowledge of growth dynamics.We have found, that a low content of membrane sterols is a characteristic of cells with suppressed proliferation activity, but necessary for high fermentation activity. Also, a high content of membrane sterols seems to enhance the viability and survival capability of harvested and dryed baker's yeast cells. Therefore, we recommend harvesting cells at a point of medium content of membrane sterols.
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