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  • 1
    ISSN: 1040-452X
    Keywords: Oocyte maturation ; Developmental capacity ; Culture medium ; Fertilization ; Mouse ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: These experiments were done to determine whether the culture medium used for the spontaneous maturation of mouse oocytes can affect the subsequent capacity of the ova to become fertilized and complete preimplantation development in vitro and development to live young. Oocytes obtained from antral follicles of gonadotropin-primed immature mice underwent spontaneous maturation in control medium, i.e. Eagle's Minimum Essential Medium (MEM) supplemented with 5% fetal bovine serum, or in one of eight different media which were also supplemented with serum. All of the ova were fertilized in Whitten's medium and were assessed for cleavage to the 2-cell stage and for further preimplantation development to blastocysts during culture in Whitten's medium. Three of the eight media used for oocyte maturation improved the capacity of the ova to develop to the blastocyst stage when compared with the control: Waymouth MB 752/1, MEM with non-essential amino acids, and MEM Alpha; Waymouth medium promoted the highest frequency of development of ova to the blastocyst stage. Moreover, the blastocysts derived from oocytes that matured in Waymouth medium contained more cells than blastocysts derived from oocytes that matured in control medium. Although BGJb medium promoted the cleavage of eggs to the 2-cell stage when present during oocyte maturation, it had a detrimental effect on their subsequent preimplantation developmental capacity. Following transfer to foster mothers, more 2-cell stage embryos developed to live young after oocyte maturation in Waymouth medium (21%) than in control medium (13%). It is concluded that the medium used for oocyte maturation in vitro can affect processes involved in the subsequent development of the eggs and that, of the media tested, Waymouth MB 752/1 promoted the highest capacity for embryo development of maturing mouse oocytes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 21 (1988), S. 409-423 
    ISSN: 0148-7280
    Keywords: meiosis ; meiotic arrest ; sterility mutation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A new murine mutation, skeletal fusions with sterility, sks, has been identified. This mutation causes arrest during the pachytene stage of virtually all spermatogenic cells. Defects in chromosome pairing and appearance of the synaptonemal complex during meiosis in the male are apparent, but defective pairing is probably not the cause of sterility. Affected females are functionally infertile. Oocytes are capable of undergoing meiotic maturation in vitro but cannot be fertilized in vitro. Affected individuals of both sexes are characterized by fusions of vertebrae and of ribs. The sks gene has been mapped to Chromosome 4, 16.6 cM distal to the brown locus.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 369 (1986), S. 847-847 
    ISSN: 1435-2451
    Keywords: Abdominal aortic aneurysm ; Diagnosis ; Indication ; Operations ; Bauchaortenaneurysma ; Diagnose ; Indikation ; Operation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An der II. Chirurgischen Abteilung des AK Harburg wurden in 5 Jahren (1981–1985) 263 Patienten mit einem infrarenalen Bauchaortenaneurysma operiert. Im vorliegenden Film werden die Diagnostik beim Bauchaortenaneurysma, die Untersuchungstechnik und -taktik beim Hausarzt und in der Klinik vorgestellt sowie die Operation zweier Aneurysmen gezeigt. Postoperative Probleme, Risikofaktoren und Kontraindikationen zur Operation werden diskutiert.
    Notes: Summary At the 2nd Surgical Department of the Harburg General Hospital in Hamburg, Germany, 263 patients with infrarenal abdominal aortic aneurysms were operated on during a 5-year period from 1981 to 1985. The film demonstrates diagnostic procedures and evaluation techniques, as employed by the family physician and the clinician. Moreover, operations for two abdominal aneurysms are shown. Postoperative problems, risk factors, and contraindications against operative procedures are discussed.
    Type of Medium: Electronic Resource
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