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  • Cranioplasty  (1)
  • HAQ  (1)
  • Kraniotomie  (1)
  • Life sciences  (1)
  • 2015-2019  (3)
  • 2010-2014  (1)
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  • 2015-2019  (3)
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  • 1
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Protein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability ́€“ FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for Îł-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles.℗
    Abstract: This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.℗
    Pages: XI, 352 p. 82 illus., 63 illus. in color. : online resource.
    ISBN: 9781493973668
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  66. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20150607-20150610; Karlsruhe; DOCMO.14.02 /20150602/
    Publication Date: 2015-06-03
    Keywords: Cranioplasty ; Titanium ; Polymethylmethacrylate ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 3
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  44. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh); 30. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh); 26. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR); 20160831-20160903; Frankfurt am Main; DOCRA.01 /20160829/
    Publication Date: 2016-08-29
    Keywords: Rheumatoide Arthritis ; Depression ; Angststörungen ; HADS ; HAQ ; Schmerz ; ddc: 610
    Language: German
    Type: conferenceObject
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  • 4
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  63. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie (JNS); 20120613-20120616; Leipzig; DOCP 105 /20120604/
    Publication Date: 2012-06-05
    Keywords: Craniotomy ; Phenytoin ; Lacosamide ; Kraniotomie ; Phenytoin ; Lacosamid ; ddc: 610
    Language: English
    Type: conferenceObject
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