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  • 1
    ISSN: 1432-2242
    Keywords: Hybrid identification ; Molecular evolution ; Repetitive DNA elements ; Satellite DNA ; Solanaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species-specific repetitive DNA probes are a useful tool for the molecular identification of somatic hybrids. Therefore, the distribution of three repetitive DNA elements of Solanum was investigated in Solanum wild species, Solanum breeding lines, and in more distantly related species of the genera Lycopersicon, Nicotiana, and Datura. The clone pSCH15, obtained from S. circaeifolium, represents a new 168-bp repetitive element; it shows 73–79% sequence similarity to repetitive elements of S. brevidens and Lycopersicon species. The 163-bp element in pSBH6, cloned from S. bulbocastanum, turned out to be very similar (95% sequence homology) to the Lycopersicon element pLEG15/TGRI previously regarded to be present only in species of the genus Lycopersicon and in S. lycopersicoides. Lower sequence similarity of approximately 80% was observed to repetitive elements of S. brevidens which are organized differently. The repeats exhibited different degrees of specificity: by Southern hybridization the element represented by the clone pSBH6 could be detected in almost all Solanum species investigated here but only after long exposure to X-ray film. The previously described “Solanum-specific” element represented by the clone pSA287 was also found, although in a very low copy number, in Lycopersicon esculentum. Therefore, detection of the repetitive elements pSA287 and pSBH6 in those species in which the respective repeat is less represented depends on exposure time. In contrast, the element pSCH15 is prominently present only in a small number of Solanum wild species and — to some extent — in the diploid breeding lines as revealed after long exposure. Use of these repeated elements for the identification of specific genomes in protoplast-fusion hybrids between Solanum wild species and Solanum breeding lines, or between two breeding lines, was evaluated.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1437-1596
    Keywords: Livor mortis ; Blanching ; Colorimetry ; Measurement of pressure ; Estimation of time of death ; Totenflecke, Wegdrückbarkeit ; Totenflecke, Farbmessung ; Totenflecke, Druckmessung ; Todeszeitschätzung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Bislang wurde die „Wegdrückbarkeit” der Totenflecken bei der Todeszeitschätzung im wesentlichen nach subjektiven Eindrücken beurteilt-danach, ob die Totenflecke leicht, vollständig, schwer und/oder unvollständig wegdrückbar sind. Ein neues Meßsystem objektiviert die Beziehungen zwischen Kraftaufwand und Farbänderung der livores („Wegdrückbarkeit”) durch digitale Verarbeitung von Meßgrößen. Der Kraftaufwand während des Drucks auf den Totenfleck wird mittels Dehnungsmeßstreifen elektronisch erfaßt. Bei Erreichen definierter Druckstärken (10, 20, 30 bis 100 Newton) löst ein Computerprogramm jeweils eine Farbmessung mit einem handelsüblichen Farbdifferenz-Meßgerät aus. Die Farbmetrik arbeitet nach dem Dreibereichsverfahren. Alle Parameter werden ineinem Meßvorgang erfaßt und über einen Meßkonverter in einen Computer eingelesen. Die Darstellung des Farborts erfolgt im L,a b-System (CIELAB nach DIN 5033, DIN 6174), das der Physiologie des Sehens sehr nahekommt. Außerdem können Farbveränderungen über eine Farbabstandsformel (ΔE) weitgehend unabhängig vom Grundton der Haut analysiert werden. In ersten Ergebnissen von Messungen an Leichen zeigen sich regelhafte postmortale Abläufe der Farbveränderungen von Totenflekken bei zunehmender Druckstärke.
    Notes: Summary Until now, blanching of livor mortis in estimating time of death has generally been assessed based on subjective impressions, i.e. on whether blanching is visible after the application of pressure. We have developed a measuring system that uses digital processing to objectify the relationship between the pressure applied and blanching of postmortem lividity. The pressure is electronically registered by a strain gauge. At predefined levels (10, 20, 30, up to 100N) the software triggers a color measurement by a commerically available tristimulus colorimeter. All parameters are measured in a single procedure and routed to the computer through a data interface. The pressure-induced color changes in the livor mortis are evaluated according to the L*, a*, b* system (CIE-LAB according to DIN 5033, DIN 6174), which closely approximates the physiology of sight. An additional color spacing formula (AE) allows analysis of color changes irrespective of the basic skin tone. Initial measurements on cadavers showed that application of increasing pressure produced regular courses of color changes in livor mortis.
    Type of Medium: Electronic Resource
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