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  • MEMBER  (3)
  • 1
    Keywords: EXPRESSION ; Germany ; human ; CDNA ; GENE ; GENES ; HYBRIDIZATION ; PROTEIN ; PROTEINS ; transcription ; FAMILY ; TRANSCRIPTION FACTOR ; primary ; DOMAIN ; BINDING ; MEMBER ; MEMBERS ; SEQUENCE ; SEQUENCES ; chromosome ; MOUSE ; TRANSCRIPTION FACTORS ; IDENTIFICATION ; IN-SITU ; AMPLIFICATION ; PROMOTER ; ELEMENTS ; HEAT-SHOCK ; DATABASE ; REGION ; FIBER ; REGIONS ; keratin ; isolation ; DOMAINS ; GENE DOMAIN ; FOLLICLE ; HAIR-FOLLICLES ; CLUSTER ; HUMAN TYPE-I ; PSEUDOGENES ; CALCIUM-BINDING PROTEIN ; HOXC13 ; cDNA,gene expression,hair follicle,in situ hybridization,keratin ; CYSTEINE-RICH PROTEINS ; HUMAN-CHROMOSOME 21
    Abstract: Analysis of the EBI/GeneBank database using nonhuman hair keratin associated protein (KAP) gene sequences as a query resulted in the identification of two human KAP gene domains on chromosome 21, one of which, located at 21q22.1, has recently been characterized. The second domain presented here, an approximately 90 kb domain on chromosome 21q23, harbored 16 KAP genes and two KAP pseudogenes. By comparison with known sheep and mouse KAP families, these genes could be assigned to two KAP families, KAP10 and KAP12, with the KAP10 family (12 members) being distinctly larger than the KAP12 family (four members). Systematic cDNA/3' rapid amplification of cDNA ends isolation studies using human scalp mRNA led to the identification of eight KAP10 and two KAP12 cDNA sequences. In situ hybridization analyses of human anagen hair follicles using specific 3'-noncoding sequences of the various KAP10/KAP12 genes revealed mRNA expression of nearly all KAP10 and KAP12 members exclusively in a narrow region of the middle portion of the hair fiber cuticle. Bioinformatic analyses of the promoter regions of the KAP10/KAP12 genes demonstrated several enhancer elements that were present in nearly all of the KAP genes. Primary among these were binding elements for the ETS, heat shock factor, AML, and HOX families of transcription factors
    Type of Publication: Journal article published
    PubMed ID: 14962103
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  • 2
    Keywords: RECEPTOR ; CELLS ; EXPRESSION ; GROWTH ; CELL ; Germany ; human ; PROSTATE ; TOOL ; GENE ; DIFFERENTIATION ; COMPLEX ; COMPLEXES ; MOTIFS ; FAMILY ; MARKER ; BINDING ; MEMBER ; MEMBERS ; SEQUENCE ; TYPE-1 ; TARGET ; ELEMENT ; ASSAY ; VECTOR ; MOBILITY ; PROMOTER ; ELEMENTS ; keratin ; RESPONSIVE ELEMENT ; FOLLICLE ; HAIR-FOLLICLES ; HUMAN SKIN ; CONSTITUTIVE EXPRESSION ; HUMAN TYPE-I ; ABSENCE ; MOTIF ; keratins ; HAIR FOLLICLE ; HOMOLOGY ; adrogens ; ANTIGEN GENE ; DERMAL PAPILLA CELLS ; IMMUNOCYTOCHEMICAL LOCALIZATION
    Abstract: Previous work had shown that most members of the complex human hair keratin family were expressed in terminal scalp hairs. An exception to this rule was the type I hair keratin hHa7, which was only detected in some but not all vellus hairs of the human scalp (Langbein et al, 1999). Here we show that hHa7 exhibits constitutive expression in medullary cells of all types of male and female sexual hairs. Medullated beard, axillary, and pubic hairs arise during puberty from small, unmedullated vellus hairs under the influence of circulating androgens. This suggested an androgen-controlled expression of the hHa7 gene. Further evidence for this assumption was provided by the demonstration of androgen receptor (AR) expression in the nuclei of medullary cells of beard hairs. Moreover, homology search for the semipalindromic androgen receptor-binding element (ARE) consensus sequence GG(A)/(T)ACAnnnTGTTCT in the proximal hHa7 promoter revealed three putative ARE motifs. Electrophoretic mobility shift assays demonstrated the specific binding of AR to all three hHa7 AREs. Their function as AR-responsive elements, either individually or in concert within the hHa7 promoter, could be further confirmed by transfection studies with or without an AR expression vector in PtK2 and prostate PC3-Arwt cells, respectively in the presence or absence of a synthetic androgen. Our study detected hHa7 as the first gene in hair follicle trichocytes whose expression appears to be directly regulated by androgens. As such, hHa7 represents a marker for androgen action on hair follicles and might be a suitable tool for investigations of androgen-dependent hair disorders
    Type of Publication: Journal article published
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  • 3
    Keywords: GLAND ; GENE FAMILY ; human ; FAMILY ; GENE ; MEMBER ; keratin ; FAMILIES
    Type of Publication: Meeting abstract published
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