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  • Basement membranes  (1)
  • Map-based gene cloning  (1)
  • Nerve cord condensation  (1)
  • Springer  (3)
  • 1
    ISSN: 1432-2242
    Keywords: Key words BAC library ; Landmark for physical mapping ; Map-based gene cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To facilitate construction of physical map of the rice genome, a bacterial artificial chromosome (BAC) library of IR64 genomic DNA was constructed. It consists of 18 432 clones and contains 3.28 rice genomic equivalents. The insert size ranged from 37 to 364 kb with an average of 107 kb. We used 31 RFLP markers on chromosome 4 to screen the library by colony hybridization. Sixty eight positive clones were identified with 2.2 positive clones per RFLP marker. The positive clones were analyzed to generate 29 contigs whose sizes ranged from 50 to 384 kb with an average of 145.6 kb. Chromosome walking was initiated for ten contigs linked to resistance genes. Thirty eight BAC clones were obtained and two contigs were integrated. Altogether, they covered 5.65 Mb (15.1%) of chromosome 4. These contigs may be used as landmarks for physical mapping of chromosome 4, and as starting points for chromosome walking towards the map-based cloning of disease resistance genes which were located nearby.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-041X
    Keywords: Drosophila ; Collagen ; Haemocytes ; Basement membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report a direct examination of the expression of one collagen gene (DCg1) during Drosophila melanogaster metamorphosis, based on data from in situ hybridization. The transcripts of this gene, thought to encode a basement membrane type IV collagen, are mainly accumulated during ecdysis in wandering haemocytes. Our results demonstrate that haemocytes contribute to extracellular matrix deposition and seem to perform a fibroblastic function during Drosophila development.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-041X
    Keywords: Type IV collagen ; Drosophila ; Germ band retraction ; Dorsal closure ; Nerve cord condensation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A collagen gene (Dcg1) was characterized in Drosophila melanogaster and shown to encode a peptide related to vertebrate basement membrane type IV collagen chains. To study the function of type IV collagen during Drosophila development, we transformed flies with a partially truncated Dcg1 gene under the control of a heat-shock promotor. This construct induced synthesis of shortened pro-α chains which associated with normal ones and thereby caused degradation of the shortened and normal pro-α chains through a process called “pro-collagen suicide”. A large proportion of embryos expressing the transgene developed a phenotype exhibiting absence or partial retraction of the germ band with defects in nerve cord condensation and dorsal closure. Together these results indicated that, during embryogenesis, type IV collagen was an essential guiding factor for cell-matrix interactions in morphogenetic events.
    Type of Medium: Electronic Resource
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