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  • Microsatellites  (3)
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  • 1
    ISSN: 1432-2242
    Keywords: Key words Barley ; Microsatellites ; Linkage map ; Genetic similarity (GS) ; Polymorphism information content (PIC)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL microsatellites were mapped either to the interspecific barley map Lerche×BGRC41936 (L×41), the Igri×Franka map (I×F, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Genotyping ; Microsatellites ; Potato ; Tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The potential of microsatellite markers for use in genetical studies in potato (Solanum tuberosum) was evaluated. Database searches revealed that microsatellite sequences were present in the non-coding regions of 24 potato genes. Twenty-two sets of primers were designed and products successfully amplified using 19 primer pairs. These were tested against a panel of 18 tetraploid potato cultivars. Four pairs of primers designed to amplify microsatellites from tomato were also used. Seven (including 2 of the tomato sequences) failed to reveal any variation in the accessions tested. Sixteen primer pairs did reveal polymorphism, detecting between 2 and 19 alleles at each locus. Of these, 3 gave rise to complex band patterns, suggesting that multiple polymorphic loci were being amplified using a single primer pair. Heterozygosity values ranged from 0.408 to 0.921. Phenetic analysis of the derived information allowed a dendrogram to be constructed depicting the relationships between the 18 potato cultivars. The potential of microsatellite markers for genetic analysis and satutory applications in potato is discussed in the context of these results. Furthermore, the potential of ‘cross-species amplification’ is highlighted as an additional source of microsatellite markers for genetic research in potato.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Genotyping ; Microsatellites ; Potato ; Tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The potential of microsatellite markers for use in genetical studies in potato (Solanum tuberosum) was evaluated. Database searches revealed that microsatellite sequences were present in the non-coding regions of 24 potato genes. Twenty-two sets of primers were designed and products successfully amplified using 19 primer pairs. These were tested against a panel of 18 tetraploid potato cultivars. Four pairs of primers designed to amplify microsatellites from tomato were also used. Seven (including 2 of the tomato sequences) failed to reveal any variation in the accessions tested. Sixteen primer pairs did reveal polymorphism, detecting between 2 and 19 alleles at each locus. Of these, 3 gave rise to complex band patterns, suggesting that multiple polymorphic loci were being amplified using a single primer pair. Heterozygosity values ranged from 0.408 to 0.921. Phenetic analysis of the derived information allowed a dendrogram to be constructed depicting the relationships between the 18 potato cultivars. The potential of microsatellite markers for genetic analysis and satutory applications in potato is discussed in the context of these results. Furthermore, the potential of ‘crossspecies amplification’ is highlighted as an additional source of microsatellite markers for genetic research in potato.
    Type of Medium: Electronic Resource
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