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  • 1
    ISSN: 1572-8773
    Keywords: cobalt binding ; cobalt-resistant ; cobalt uptake ; metal-resistant ; N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A cobalt-resistant strain of Neurospora crassa (cor) was obtained by repeated subculturing of the wild type on cobalt-containing agar medium. N. crassa cor is twentyfold more resistant to cobalt ions compared with the wild type. Resistance was stable on repeated subculturing of cor on cobalt-free media. N. crassa cor is also cross-resistant to nickel (fourfold), but not to zinc or copper. Higher concentrations of iron and magnesium ions are required to reverse growth inhibition due to cobalt toxicity in N. crassa cor, compared with the wild type. Germinating conidia and mycelia of the cor strain accumulated lower levels of cobalt ions compared with the parent N. crassa. The partial transport block for cobalt uptake is shown to be primarily due to decreased surface binding of cobalt to mycelia and cell walls. Efflux of mycelial cobalt was also observed in wild type and cobalt-resistant N. crassa. The characteristics of cor in comparison with wild type N. crassa are discussed in relation to the mechanisms of cobalt resistance.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-8773
    Keywords: cobalt-binding ; cobaltoprotein ; cobalt-resistant ; metal-resistant ; N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A cobalt-resistant strain of Neurospora crassa (cor) is 20-fold more resistant to Co when compared with the wild type. DEAE-cellulose and metal-chelate affinity chromatography of cell-free extracts separated cobalt into protein-bound and free ionic fractions. In N. crassa cor about 80% cobalt of cell-free extracts was protein bound while the same in wild type was only 25%. Cobalt content of the protein-bound fraction increased with time and cobalt concentration in the growth medium, and was not influenced by related metal ions. A cobaltoprotein (CBP) which is overproduced in N. crassa cor and constitutes up to 12% of total protein of extracts was purified. CPB is a brown coloured (absorption peaks at 275, 350 and 440nm), small molecular weight glycoprotein (Mr 8200 daltons) with 28 - 30% carbohydrate (mannose). CBP has 70 μg cobalt mg-1 protein. Cysteine, glycine, glutamic acid and aspartic acid are the major amino acid constituents. The role of CBP in cobalt resistance is discussed in relation to other known metalloproteins involved in resistance. © Rapid Science 1998.
    Type of Medium: Electronic Resource
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