Life and Medical Sciences
Cell & Developmental Biology
Wiley InterScience Backfile Collection 1832-2000
The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, PI1, PI2, and lamin B) were used to immunolocalize nuclear matrix antigens in germinal vesicle oocytes, metaphase II oocytes, zygotes, two-cell-stage embryos, and eight-cell stage embryos. All antibodies reacted with the nuclei of germinal vesicle oocytes, and two- and eight-cell-stage embryos; however, only P1 and lamin B were present at the pronuclear stage. In eggs collected at the pronuclear stage and cultured to the late two-cell stage in the presence of α-amanitin, the matrix morphology was altered for PI1 and PI2. α-Amanitin had no affect on the distribution of P1 or lamin B antigens. If α-amanitin was added 2 hr after cleavage to the two-cell stage, the normal staining pattern of PI2 was retained. These results suggest that the presence of specific components of an internal matrix is correlated with normal genomic activity.
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