Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract The black blowfly,Phormia regina, has been implicated in human myiasis and as a contact vector of viral and bacterial diseases present in carrion to which female flies are attracted for egg deposition. Inbred strains ofP. regina are an excellent model system for studying gene expression in the developmental stages of such holometabolous dipteran parasites. However, information regarding gene and protein expression patterns inP. regina is limited. We used ISO-DALT high-resolution, two-dimensional electrophoresis with silver staining to establish fundamental protein maps for examination of the stage-specific gene expression patterns in the 615 most abundant proteins of the eggs, first- and third-instar larvae, pupae, and male and female adults. We also used a differential extraction technique to identify the major cuticular proteins of the adults. The results show 48 clearly identifiable stage-specific and sex-specific proteins. Thus, approximately 8% of the most abundant proteins exhibit developmental changes. These analyses serve as an initial data base for further studies of ontogenetic regulation, organellar origin, and physiologic function of the stage-specific proteins in the life cycle of these opportunistically parasitic dipterans.
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