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  • Polymer and Materials Science  (9)
  • thermal diffusivity  (3)
  • DOWN-REGULATION  (2)
  • 1
    Keywords: RECEPTOR ; KINASE ; PROTEIN ; DOWN-REGULATION ; fibroblasts ; BONE-MARROW ; STROMAL CELLS ; INTERNATIONAL-SOCIETY ; THERAPY POSITION STATEMENT ; GENOME-WIDE RNAI
    Abstract: Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting.
    Type of Publication: Journal article published
    PubMed ID: 26120366
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  • 2
    Keywords: EXPRESSION ; MICE ; DOWN-REGULATION ; METHYLATION ; METHYLTRANSFERASE ; NEPHROGENIC DIABETES-INSIPIDUS ; AQUAPORIN-2 TRAFFICKING ; WATER CHANNELS ; ENAC-ALPHA ; SET DOMAIN
    Abstract: Dot1l encodes histone H3 K79 methyltransferase Dot1a. Mice with Dot1l deficiency in renal Aqp2-expressing cells (Dot1l(AC)) develop polyuria by unknown mechanisms. Here, we report that Aqp5 links Dot1l deletion to polyuria through Aqp2. cDNA array analysis revealed and real-time RT-qPCR validated Aqp5 as the most upregulated gene in Dot1l(AC) vs. control mice. Aqp5 protein is barely detectable in controls, but robustly expressed in the Dot1l(AC) kidneys, where it colocalizes with Aqp2. The upregulation of Aqp5 is coupled with reduced association of Dot1a and H3 dimethyl K79 with specific subregions in Aqp5 5' flanking region in Dot1l(AC) vs. control mice. In vitro studies in IMCD3, MLE-15 and 293Tcells using multiple approaches including real-time RT-qPCR, luciferase reporter assay, cell surface biotinylation assay, colocalization, and co-immunoprecipitation uncovered that Dot1a represses Aqp5. Human AQP5 interacts with AQP2 and impairs its cell surface localization. The AQP5/AQP2 complex partially resides in the ER/Golgi. Consistently, AQP5 is expressed in none of 15 normal controls, but in all of 17 kidney biopsies from patients with diabetic nephropathy. In the patients with diabetic nephropathy, AQP5 colocalizes with AQP2 in the perinuclear region and AQP5 expression is associated with impaired cellular H3 dimethyl K79. Taken together, these data for the first time identify Aqp5 as a Dot1a potential transcriptional target, and an Aqp2 binding partner and regulator, and suggest that the upregulated Aqp5 may contribute to polyuria, possibly by impairing Aqp2 membrane localization, in Dot1l(AC) mice and in patients with diabetic nephropathy.
    Type of Publication: Journal article published
    PubMed ID: 23326416
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  • 3
    ISSN: 1042-7147
    Keywords: Polymer blends ; Crystallization ; Relaxation behavior ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this paper we focus on miscible blends of two engineering polymers: poly(butylene terephthalate) (PBT) and a polyarylate (PAr). The issue of transesterification in these blends will be addressed, followed by a discussion of the crystallization kinetics of PBT, poly(ethylene terephthalate) and several PBT/PAr blends. The ability to estimate polymer-polymer interaction parameters in blends from melting point depression will also be discussed. The amorphous phase behavior of the PBT/PAr blends has been explored primarily using dielectric spectroscopy. For blends in which PBT has crystallized, we observe two relaxations associated with Tg-like motion, and this behavior is interpreted in light of our recent work on order-disorder interphases in crystalline blends.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    ISSN: 0887-6266
    Keywords: polymer solution ; light scattering ; poly(isobornyl methacrylate) ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Thermodynamic and hydrodynamic properties of dilute solutions of poly (isobornyl methacrylate) (PIMA) in tetrahydrofuran (THF) were characterized by using viscosity, static, and dynamic light scattering measurements. PIMA samples with different molecular weight were obtained by fractional precipitation of PIMA solution. Chain dimension parameters (Rg and RH), together with second virial coefficient A2 and intrinsic viscosity [η], were used to calculate various solution parameters characterizing polymer chains in polymer solutions. The experimental results are compared with calculation, indicating that PIMA behaves as a flexible coil in THF. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A series of hydroxyl-terminated maleopimaric acid esters (HTMAEs) and rigid polyurethane (PU) foams based on these HTMAEs were synthesized using chemically modified natural gum rosin and its derivative maleopimaric acid as raw materials. Thermal stability of these polyols and their corresponding rigid PU foams was studied by a thermogravimetric method and a dimensional stability measurement. It was shown that the thermal stability of the final foams was strongly dependent on the structure of their corresponding polyols. The thermogravimetric analysis curves of these rosin-based rigid PU foams displayed two distinct regions of weight loss. It has been shown that at the initial stage of weight loss the process was dominated by polyol component degradation; the second stage was governed by isocyanate component degradation. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A series of rosin-based rigid polyurethane foams of different composition were synthesized directly from chemically modified gum rosin. The effect of the composition of these rosin-based rigid polyurethane foams on their thermal stability and compression strength was measured. It was shown that the onset temperature of weight loss and the dimensional stability at high temperature increased with increase of the molar ratio of NCO/OH. The TGA data further confirmed that the second stage weight loss in the two-stage weight loss process of these polyurethane foams was governed by thermal degradation of the isocyanate component. Although density had no significant influence on the TGA curves of the rosin-based rigid polyurethane foams, it had great influence on the dimensional stability at high temperature and compression strength of the foams. It has been shown that the inclusion of rosin in rigid polyurethane foams increases the strength and thermal stability compared with that of polyether-based ones. © 1996 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    ISSN: 0887-6266
    Keywords: physical aging ; tracer diffusion coefficient ; camphorquinone ; polysulfone ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The effect of physical aging on the tracer diffusion coefficient D of camphorquinone in polysulfone is investigated. It is shown that if the sample is sufficiently annealed and physical aging is nearly complete, the temperature dependence of D will reflect the primary α-relaxation process of the host polymer. In the temperature range between Tg (=185°C) and 165°C, D is found to be a function of time, and the time dependence of D is given by D = At-μ, with μ approximately equal to unity. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: In order to obtain quantitative surface compositional information, an earlier crystallographic electron attenuation model has been extended to treat polycrystalline alloys. Simple correction factors are obtained that yield large corrections to elemental Auger intensity ratios. The model has been applied to a binary surface alloy formed by heating cerium overlayers on a polycrystalline rhodium substrate. It is shown that an alloy film is formed whose thickness is proportional to the number of Ce layers initially deposited. The surface alloy is identified as Ce3Rh2, which corresponds to a known bulk phase.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Surface modification of polypyrrole (PPY), polyaniline (PAN) and poly(3-alkylthiophene) films by ozone was studied by angle-resolved x-ray photoelectron spectroscopy (XPS). In the case of the N-containing polymers, oxidation occurs readily and mainly at the carbon atoms, resulting in the formation of CνO, CξO, COOH and even OνCOOH (especially for PAN) species. The carbon atoms of doped PPY and PAN films are significantly more resistant to ozone oxidation, but the samples suffer some loss of the dopant in the surface region. Ozone treatment does not involve the direct oxidation of the nitrogen heteroatoms to form theνNOx species, even at high extent of carbon oxidation. In addition, a substantial decrease in the intrinsic oxidation state ([ξNν]/[νNHν] ratio) was observed in the 25% deprotonated PPY (DP-PPY) base, the 50% intrinsically oxidized emeraldine (EM) base and the 75% intrinsically oxidized nigraniline (NA) base after ozone treatment and subsequent atmospheric exposure. In the case of the S-containing polymers, ozone treatment results predominantly in the oxidation of sulphur heteroatoms to give rise to the sulphone and peroxide species. The oxidized sulphur species, however, are readily reduced or consumed in a photochemical reaction.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1572-9567
    Keywords: composites ; copper ; flash method ; iron ; thermal diffusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A method for measuring thermal diffusivity in one of the layers of a two-layer composite sample has been described. The heat transfer problem of a two-layer sample associated with pulse thermal diffusivity measurements has been analyzed for two cases: exponential and square-wave pulses. According to our measurements, a triangular heat-pulse function approximates reasonably well the output of the Nd-glass laser. In this paper, an expression is derived for the temperature transient at the rear face of two-layer sample being subjected to a triangular heat-pulse input on the front face. The analytical solution of the problem forms the basis of our method of data reduction. This solution has been programmed for computer processing of the data. The method described here has been successfully tested by limited measurements on copper and iron.
    Type of Medium: Electronic Resource
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