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  • Protein A-peroxidase  (2)
  • AIDS  (1)
  • Polymorphonuclear leukocytes
  • 1
    ISSN: 1432-069X
    Keywords: Protein A-peroxidase ; Immuno-electron microscopy ; Pemphigus ; Protein A-Peroxidase ; Immunelektronenmikroskopie ; Pemphigus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Pemphigus-Autoantikörper in der Epidermis wurden während verschiedener Stadien der fortschreitenden Acantholyse mit einer neuen immunelektronenmikroskopischen Technik dargestellt. Peroxidase-markiertes Protein A wurde als ein neuer und spezifischer Tracer für gewebegebundene Antikörper vom Typ IgG eingesetzt. Hierbei zeigten sich als Vorteile dieses neuen Nachweisreagenz 1. sein relativ geringes Molekulargewicht, 2. seine Fähigkeit zur raschen Gewebepenetration, und 3. die durch verkürzte Inkubationszeiten verbesserte Erhaltung der feingeweblichen Struktur. Die unspezifische Adsorption im Gewebe und auf Zellen war vergleichsweise gering.
    Notes: Summary Pemphigus autoantibodies, bound in the epidermis during different stages of acantholysis, were demonstrated with a new techniques for immunoelectron microscopy. Peroxidase-labeled Protein A was used as a new and specific tracer for tissue-bound antibodies of the IgG-type. Advantages were: (1) A small molecular weight of the tracer, (2) a rapid tissue penetration, and (3) shortened incubation times, thus better preserved tissue fine structures. Unspecific adsorption in tissues and on cells was found to be comparatively low.
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  • 2
    ISSN: 1432-069X
    Keywords: Kaposi's sarcoma ; Initial lesion ; HIV infection ; Spindle cells ; AIDS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Kaposi's sarcoma (KS) in human immunodeficiency virus infection (HIV) has become a rather frequent manifestation of the previously rare disease with fatal outcome. Initial lesions of KS were studied by means of histopathology, immunohistology, and electron microscopy in order to define the earliest alterations. The histopathological changes of initial lesions were distinct, consisting of (1) discrete proliferation of capillary vessels, (2) dissection of collagen by proliferating spindle cells which formed slits, (3) atypical spindle cells arranged in an Indian file pattern, and (4) the lack of any inflammatory cellular infiltrate. Double staining with antibodies against vimentin and immunohistochemical markers for endothelial cells revealed that slits forming vimentin-positive spindle cells displayed laminin, factor VIII, and PAL-E. Atypical vimentinpositive spindle cells arranged in an Indian file pattern inconsistently expressed laminin and factor VIII, but not PAL-E. KS cells rarely stained with the lectin UEA I, not even in case of less advanced dedifferentiation. Electron microscopy showed gradual transformation between spindle cells forming slits and those having lost the ability to form incomplete vessel walls. The present findings support the view that KS develops from the endothelial cells of the blood vessels. The proliferation of atypical endothelial cells as early as in initial lesions and the lack of inflammation favors the primary neoplastic genesis of KS.
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  • 3
    ISSN: 1432-069X
    Keywords: Psoriasis ; Polymorphonuclear leukocytes ; Myeloperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The specific activity of myeloperoxidase (MPO), an enzyme located in the primary granules of polymorphonuclear leukocytes, was measured in patients with psoriasis vulgaris and compared with that in patients with atopic dermatitis and in healthy subjects. MPO catalyzes the oxidation of guaiacol into tetraguaiacol in the presence of H2O2. The activity was determined by photometric measurement of tetraguaiacol. The specific MPO activity showed no statistically significant difference between the healthy subjects and the patients with atopic dermatitis. In comparison to these two groups the specific MOP activity of the psoriatic patients showed a slight reduction which was, however, not statistically significant.
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  • 4
    ISSN: 1432-1440
    Keywords: Protein A-Peroxidase ; Immunohistologie ; Immunoenzymtechnik ; Antinukleäre Antikörper ; Protein A-peroxidase ; Immunohistology ; Immunoenzyme technique ; Antinuclear antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Protein A from Staphylococcus aureus, a cell wall protein with high affinity binding properties for IgG-type antibodies, has been labeled with peroxidase to form a stable immunohistological tracer molecule of relatively low molecular weight. It has been used for demonstration and titration of antinuclear antibodies in SLE sera on mouse liver sections in an indirect technique. The findings were consistent with those obtained by immunofluorescence and by staining with peroxidase-coupled anti-IgG. In contrast to immunofluorescence, the stained sections could be mounted and stored for documentation. In comparison, unspecific tissue adsorption and staining could be minimized by addition of glucose, galactose, and mannose as well as bovine serum albumine to the buffer containing Protein A-peroxidase.
    Notes: Zusammenfassung Protein A von Staphylococcus aureus, ein Zellwandprotein mit hoher Affinität für Antikörper vom Type IgG, wurde mit Peroxidase markiert. Es entstand ein stabiles Tracer-Molekül von relativ niedrigem Molekulargewicht für den immunohistologischen Nachweis von gewebsgebundenen Antikörpern. In einer indirekten Technik wurden auf Mäuselebergewebe antinukleäre Antikörper aus Seren von Patienten mit Systemischem Lupus Erythematodes nachgewiesen und titriert. Die Befunde stimmten mit denjenigen bei Immunfluoreszenz und bei Immunoenzymtechnik mit Peroxidase-markiertem anti-IgG überein. Im Gegensatz zur Immunfluoreszenz können die Präparate nach der Immunoenzymreaktion zur Dokumentation aufbewahrt werden. Die bei allen Techniken auftretende unspezifische Adsorption von Tracermolekülen im Gewebe konnte durch Zusatz von Glucose, Galactose und Mannose zur Protein A-Peroxidase-Suspension deutlich vermindert werden.
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