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  • Relaxation complex  (1)
  • 1
    ISSN: 1617-4623
    Keywords: ColE1 ; Plasmid mobilization ; Overlapping genes ; Site-directed mutagenesis ; Relaxation complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A third of the 6.6 kb genome of ColE1 is devoted to mobilization (mob) genes necessary to promote its specific transfer in the presence of conjugative plasmids. Themob region is genetically complex: twomob genes are entirely overlapped by a third. Oligonucleotide-directed mutagenesis was used to insert an amber codon into one of the overlapped genes and make possible a full complementation analysis ofmob. Fourmob genes essential for mobilization by R64drd11 were thus identified. Fragments ofmob were subcloned under control of the Ptac promoter in a suitable vector, overexpressed in minicells and the mobilization proteins visualized. A comprehensive alignment of themob region of ColE1 with those of its close relatives ColK and ColA demonstrating that the four essentialmob genes are conserved is also presented.
    Type of Medium: Electronic Resource
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