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  • Chemical Engineering  (10)
  • Saccharomyces cerevisiae  (7)
  • Wiley-Blackwell  (17)
  • 1990-1994  (17)
  • 1
    ISSN: 0749-503X
    Keywords: RVS161 gene ; Saccharomyces cerevisiae ; stationary phase entry ; viability loss ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In yeast, nutrient starvation leads to entry into stationary phase. Mutants that do not respond properly to starvation conditons have been isolated in Saccharomyces cerevisiae. Among them the rvs161 mutant (RVS for Reduced Viability upon Starvation) is sensitive to carbon, nitrogen and sulphur starvation. When these nutrients are depleted in the medium, mutant cells show cellular viability loss with morphological changes. The mutation rvs161-1 is very pleiotropic, and besides the defects in stationary phase entry, the mutant strain presents other alterations: sensitivity to high salt concentrations, hypersensitivity to amino acid analogs, no growth on lactate or acetate medium. The addition of salts or amino acid analogs leads to the same morphological defects observed in starved cells, suggesting that the gene could be implicated mainly in the control of cellular viability. The gene RVS161 was cloned; it codes for a 30,252 daltons protein. No homology was detected with the proteins contained in the databases. Moreover, Southern analysis revealed the presence of other sequences homologous to the RVS161 gene in the yeast genome.
    Additional Material: 6 Ill.
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  • 2
    ISSN: 0749-503X
    Keywords: Chromosome III ; Saccharomyces cerevisiae ; mating type ; HML ; BUD5 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: This paper reports the DNA sequence of a segment of 9·8 kb of the chromosome III. The sequenced DNA contains the MATα locus. The new sequence of the MATα locus differs from the previously reported sequence by six modifications in the W segment. We have found the same modifications in the HML locus. The corrected sequence contains, in HML, an open reading frame (ORF) of 190 codons which ends at the border between the W segment and the flanking DNA. In the MAT locus, this ORF extends in the flanking DNA up to 538 codons. This ORF corresponds to a gene independently identified as BUD5 (Chant et al., 1991). This gene presents homologies with the exchange factors SDC25 and CDC25. A large ORF of 1399 codons is found on the opposite side of MATα (toward the telomere). This ORF corresponds to a new gene YCR724. Next to this gene is a small ORF, YCR725, of 127 codons. The localization of this fragment on chromosome III, originally supposed to be distal from the MAT locus based on genetic distance, illustrates variation in recombination frequency along the chromosome and suggests the existence of hot spots of recombination between MAT and the THR4 locus.
    Additional Material: 5 Ill.
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  • 3
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; chromosome II sequencing ; serine-hydroxymethyl-transferase ; RIB5 ; GAP ; GTP binding protein ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report here the sequence of a 19,482 bp DNA segment of chromosome II of Saccharomyces cerevisiae. The fragment contains 16 open reading frames (ORFs) covering 74% of the sequence. Four predicted products present homology with known proteins. The ORF YBR1732 exhibits a strong homology to serine hydroxymethyl transferase; the best score is 53·1% identity in 458 amino acids overlap with the serine hydroxymethyl transferase from rabbit liver. YBR1724, which shows homology with riboflavin synthase of Bacillus subtilis, is probably the RIB5 gene implied in riboflavine synthesis and mapped in this region. YBR1733 is homologous to rab protein and YBR1728 is presumably a GTPase activating protein.
    Additional Material: 5 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 9 (1993), S. 495-506 
    ISSN: 0749-503X
    Keywords: Nuclear migration ; protein repeats ; cell cycle ; Saccharomyces cerevisiae ; nutrient starvation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated a mutant (rvs272) of the yeast (Saccharomyces cerevisiae) that displays an altered phenotype in stationary phase. It shows a high proportion of large-budded cells with two non-segregated nuclei staying in the mother cell. This phenotype is also expressed in various conditions when cells are synchronized, energy depleted or treated with the antimitotic drug benomyl. The RVS272 gene has been identified as the NUM1 gene already described. This gene presents a 192 bp tandemly repeated motif and we show that the number of repeats can vary from 1 to about 24 among different strains, without apparently affecting the function of the encoded protein. We suggest that this protein could be involved in polymerization catalysis and/or stabilization of microtubules.
    Additional Material: 8 Ill.
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  • 5
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; HSP26 ; SEC18 ; UBC4 ; tRNAarg ; tRNAasp ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The nucleotide sequence of a 31 352 bp fragment from chromosome II of Saccharomyces cerevisiae has been determined and analysed. The fragment originates from the right arm of chromosome II, located between the GAL7,10,1 and the PHO3,5 loci, at a distance of about 130 kb from the centromere. The sequence contains a tRNA tandem repeat and 17 open reading frames (ORFs) larger than 100 amino acids. One of them extends into adjacent DNA and is incomplete. The two tRNA genes, coding for a tRNAasp and a tRNAarg, and three of the ORFs, had been sequenced previously, i.e. HSP26, SEC18, and UBC4. Four other ORFs showed similarity with yeast genes; amino acid transporter genes, the RAD54, SNF2 and STH1 family, the SPS2 gene and the bromodomain of SPT7, respectively. Two showed homology with sequences from other organisms, i.e. with a Plasmodium falciparum gene encoding a surface antigen and with a gene from Saimirine herpes virus respectively. Three ORFs, YBR0726, YBR0735 and YBR0740 are completely contained in YBR0727, YBR0734 and YBR0739 respectively, and thus probably do not represent real genes. Two ORFs, YBR0727 and YBR0745 most likely contain an intron. The sequences have been deposited in the EMBL data library under Accession Number X76294.
    Additional Material: 1 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 207-215 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An on-line estimation technique for multiple specific growth rates is proposed. Time-varying tuning parameters are used to obtain constant estimation error dynamics in spite of strongly varying process dynamics commonly encountered in bioprocesses. This approach simplifies the tuning of the algorithm to a pole placement procedure. Application of the method is illustrated through the study of the baker's yeast fedbatch process. Guidelines for selection of measured state variables are given in relation to the performance of the estimation method.
    Additional Material: 14 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 1751-1760 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The experimental validation of on-line estimation of multiple specific growth rates for the bakers' yeast fed-batch process is presented. Pole placement based parameter estimation combined with an asymptotic biomass observer constitute the basic algorithm. The full process model being ill-conditioned for estimation using the available measured state variables, the use of two partial models related to two different states of the process is suggested. An alternating procedure between two sets of estimation algorithms designed from the partial models is proposed. The performance of the alternating procedure is validated both with simulated and experimental data. The accuracy of the estimates of the three specific growth rates involved in this process is verified according to two criteria based on the respiratory quotient and on the evaluation of the ethanol production/consumption rate.
    Additional Material: 20 Ill.
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  • 8
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The transesterification reaction catalyzed by dibutyltin oxide has been used to crosslink miscible blends (at about 200°C) of ethylene vinyl acetate (EVA) and ethylene methyl acrylate (EMA) copolymers. Then, microwave heating at 2.45 GHz with the fundamental TE01 mode was used for activation of this crosslinking reaction. The microwave treatment was carried out in a continuous process through a resonant cavity on line with a twin screw extruder equipped with a strip die or a circular die, which imposed the shape of the samples in the waveguide. Therefore, a shape factor due to the geometry of the samples and their orientation with respect to the electromagnetic field was defined. Last, this technique was applied to the microwave crosslinking of an EVA/EMA blend dispersed in a polypropylene matrix and consequently offers a new route to control the morphology of the polymer blends.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 193-201 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A generalized linear dynamic model or singular model, for which the standard state space representation and the Kalman filtering cannot be applied, is used to develop a new algorithm to solve the linear dynamic material balance problem. This algorithm is based on the method developed in the steady-state case and leads to a recursive scheme, which is very useful in real-time processing. It reduces the computational problem such as singularities and round-off errors that may occur in complex systems. Convergence conditions are given and verified for the dynamic material balance case.
    Additional Material: 7 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 1449-1460 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Many industrially significant microbial products are produced during secondary metabolism by fungal pellets. Most models of mycelial pellet growth, however, consider only the primary growth phase. During secondary metabolism, the whiterot fungus Phanerochaete chrysosporium produces extra-cellular lignin peroxidases and manganese-dependent peroxidases which have been implicated in the degradation of lignin and various xenobiotics. This unstructured kinetic model for mycelial pellet cultures of P. chrysosporium describes the culture dry weight, respiration, glucose consumption, nutrient nitrogen uptake, pellet size and oxygen effectiveness factor during the lag, primary growth, secondary metabolic, and death phases. During secondary metabolism, the intraparticle diffusion of oxygen limits metabolism. Parameters for respiration during secondary metabolism are validated using three separate measurements: 1. by measuring oxygen concentration profiles in mycelial pellets using oxygen microelectrodes; 2. by measuring the depletion of oxygen from the culture fluid using a respirometer; and 3. by measuring the daily evolution of carbon dioxide. The physiological state of the mycelial pellets is predicted for various culture conditions and is related to the production of LIP and MNP during secondary metabolism.
    Additional Material: 8 Ill.
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