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  • Senecio cannabifolius  (1)
  • antifeedant bioassay  (1)
  • 1
    ISSN: 1420-9071
    Keywords: Senecio cannabifolius ; Compositae ; foliar surface chemistry ; tobacco cutworm ; Spodoptera litura ; Lepidoptera ; Noctuidae growth inhibition ; ethyl (1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl) acetate ; methyl (1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl) acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Dewaxed leaf surface extracts of 12 plants from Hokkaido, prepared by dipping fresh leaves in chloroform for 3 min, were used in a choice leaf-disk bioassay against larvae of the tobacco cutwormSpodoptera litura. Activity was found only in the extract ofSenecio cannabifolius, a very successful weed in Hokkaido. Individual fractions of the extract, however, were not active. Incorporation of the individual fractions of the surface extracts as well as fractions of the methanolic extracts of the leaf residue into an artificial diet fed to neonateS. litura led to the isolation of ethyl (1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl) acetate, the major surface compound, as the active principle. This compound was also present in the methanolic extract of the leaf residue together with methyl (1-hydroxy-4-oxocyclohexa-2,5-dien-1-yl) acetate, which had the same growth inhibitory effect on the larvae. The presence of these compounds in the foliar surface and tissue suggests a defensive role against herbivores.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1570-7458
    Keywords: video image analysis ; antifeedant bioassay ; computer interface ; Spodoptera litura ; Skimmia japonica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of our search for new insect antifeedant substances from plants, we have devised an improved antifeedant leaf-disk bioassay. Our system allows an accurate measurement of consumed disk surfaces, using a video camera interfaced with a computer. The scanned image of the leaf disks is stored, and the eaten areas are measured with the help of a video-image analysis software. This new method allows for precise quantification of insect antifeedant activity tested on leaf material. This method was applied to the screening of Hokkaido plants for antifeedant activity. Out of 206 samples, seven demonstrated significant activity against larvae of the tobacco cutworm,Spodoptera litura.
    Type of Medium: Electronic Resource
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