Springer Online Journal Archives 1860-2000
Summary The histochemical localization and activity of 5 hydrolytic and 5 oxidative enzymes in the spinal cord, spinal ganglion and muscle of the adult rat were studied at intervals of 1, 2, 3 and 4 weeks after weekly intraperitoneal (i.p.) injections of 5 mg/kg of antimetabolitic 6-aminonicotinamide (6-AN) and also 24 h after an injection of 20 mg/kg 6-AN. The rats were decapitated. One part of the spinal cord was frozen with liquid nitrogen and one part fixed in formalin. Seven days after the injection of 5 mg/kg proliferation of the glia in the center of the anterior grey matter of the spinal cord occurred accompanied by marked ATPase and acid phosphatase activity. A loss of histochemical activity in oxidative enzymes, especially of glucose-6-phosphate dehydrogenase, occurred. After the 3rd and 4th doses of 5 mg/kg i.p. the histochemical activity increased, especially in the capillaries. After a 20 mg/kg dose of 6-AN a marked decrease in activity of the oxidative and hydrolytic enzymes in the grey and white matter of the spinal cord was noted. We observed selective gliosis in the intermediate zone of the anterior horn and in rats receiving several doses of 6-AN, we also observed morphological and histochemical compensation. Striated skeletal muscle remained unchanged.
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