Springer Online Journal Archives 1860-2000
Abstract Monoclonal antibodies against germ-tube specific antigens ofCandida albicans were produced by a selective immunization protocol. The monoclonal antibody (MAb) DC3:H10 recognized a determinant preferentially expressed by germ tubes of the fungus compared to yeast cells. The MAb DC3:H10 antigen was expressed by nearly all germ tubes of the strains 3153A and B311. It was also expressed by germ tubes of the white phenotype of strain WO-1, and by many exponentially growing yeast cells of the opaque phenotype of this strain. The determinant appeared very rapidly after induction of germ tubes of strain 3153A. The MAb DC3:H10 epitope was sensitive to proteolytic treatment but not to periodate treatment, indicating its protein nature. The reactive material could be extracted from intact germ tubes by treatment with β-mercaptoethanol. On elution from a G-200 Sephadex column it yielded an apparent molecular weight of 519 kDa. This fraction appeared heterogeneous since at least two major bands of lower molecular weight were detected by silver staining following electrophoretic separation under denaturing conditions.
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