Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • cDNA  (2)
  • AC-ECD  (1)
  • Springer  (3)
  • Munksgaard International Publishers
  • 1
    ISSN: 1432-2048
    Keywords: Key words: Ascorbate peroxidase ; cDNA ; Glycine (ascorbate peroxidase)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Screening of a cDNA library from soybean (Glycine max (L.) Merr. cv. Century) with probes based upon cytosolic ascorbate peroxidase (APx; EC 1.11.1.11) genes identified two full-length clones (SOYAPx1, SOYAPx2) apparently encoding for different soybean leaf cytosolic APxs. The deduced amino acid sequences of the two APx cDNA products differed in 13 of the 250 amino acids. The SOYAPx1 cDNA was identical to the cytosolic APx cDNA previously found in soybean root nodules. Escherichia coli expression systems were developed using both soybean APx cDNAs. Recombinant SOYAPx1 and SOYAPx2 were then utilized to characterize the enzymatic properties of the two APx cDNA products.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1573-4919
    Keywords: cDNA ; human ; placenta ; immunoglobulin ; pregnancy-specific β1-glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Three cDNAs encoding members of the pregnancy-specific β1-glycoprotein (PSG) family were isolated from human term placental cDNA library. All three cDNAs encode proteins with similar domain structure. There is a leader sequence of 34 amino acids followed by an N-domain of 109 amino acids. Immediately after the N-domain are one or two copies of a repeating A-domain of 93 amino acids, a B-domain of 85 amino acids and a C-domain of variable size. The proteins are highly hydrophilic. However, one of them has an 81-amino acid C-domain which is very hydrophobic and could potentially serve as a membrane attachment site. The putative cell-cell recognition tripeptide, Arg-Gly-Asp, is present in the N-domain of two of the proteins. Partial sequence of one of the cDNAs has been found in HeLa cells while cDNAs highly homologous to two of the cDNAs have been found in the fetal liver. Functional roles of the PSG proteins basing on their structure are proposed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Microchimica acta 96 (1988), S. 11-26 
    ISSN: 1436-5073
    Keywords: electron capture detector (ECD) ; electron oscillation ; AC-ECD ; argon-methane ; non-homogeneous ECD kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The oscillation of electrons—as driven by AC polarization—can be used to extract a high-sensitivity signal from a conventional electron capture detector. For example, hexachloroethane was detected at a hypercoulometric ratio of 2.0 F/mol, down to ca. 60 fg/s (S/N=3) and with a linear range in excess of two orders of magnitude. The change in carrier gas from nitrogen to argon-methane produced the expected order-of-magnitude increase in optimum oscillation frequency. Anab initio simulation of potentials and ion populations in a heterogeneous electron capture system under a high-frequency AC regime provided further insight into the detector's mechanism: Hypercoulometric response is mainly caused by increased cation-electron recombination in the plasma region, owing to a decreased field gradient and an increased cation concentration.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...