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  • Ultrastructure  (3)
  • cell volume  (2)
  • cultured cells  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Phytochemistry 20 (1981), S. 545-547 
    ISSN: 0031-9422
    Keywords: Coptis japonica ; Ranunculaceae ; berberine production. ; cultured cells
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Phytochemistry 23 (1984), S. 281-285 
    ISSN: 0031-9422
    Keywords: Coptis japonica ; Ranunculaceae ; alkaloid ; berberine. ; biosynthesis ; cultured cells ; regulation ; selection ; small cell aggregate-cloning
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0533
    Keywords: Primary rhabdomyosarcoma ; Brain tumor ; Ultrastructure ; Basal ganglia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Primary intracranial rhabdomyosarcoma is extremely rare. A case of primary rhabdomyosarcoma in the left basal ganglia is described in a 2-year-old boy. The investigations revealed no primary lesion elsewhere except in the brain. At the electron-microscopic level, the majority of the cells contained specific microfilamentous bundles, which were either distributed at random or organized in myofibrils identified by their cross striations with primitive Z band formation.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1424
    Keywords: eccrine sweat gland ; cell volume ; X-ray microanalysis ; acetylcholine ; potassium ; sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Methacholine (MCh)-induced changes in intracellular concentrations of Na, K, and Cl ([Na]i, [K]i, and [Cl]i, respectively) and in cellular dry mass (a measure of cell shrinkage) were examined in isolated monkey eccrine sweat secretory coils by electron probe X-ray microanalysis using the peripheral standard method. To further confirm the occurrence of cell shrinkage during MCh stimulation, the change in cell volume of dissociated clear and dark cells were directly determined under a light microscope equipped with differential interference contrast (DIC) optics. X-ray microanalysis revealed a biphasic increase in cellular dry mass in clear cells during continuous MCh stimulation; an initial increase of dry mass to 158% (of control) followed by a plateau at 140%, which correspond to the decrease in cell volume of 37 and 29%, respectively. The latter agrees with the MCh-induced cell shrinkage of 29% in dissociated clear cells. The MCh-induced increase in dry mass in myoepithelial cells was less than half that of clear cells. During the steady state of MCh stimulation, both [K]i and [Cl]i of clear cells decreased by about 45%, whereas [Na]i increased in such a way as to maintain the sum of [Na]i+[K]i constant. There was a small (12–15mm) increse in [Na]i and a decrease in [K]i in myoepithelial cells during stimulation with MCh. Dissociated dark cells failed to significantly shrink during MCh stimulation. The decrease in [Cl]i in the face of constant [Na]i+[K]i suggests the accumulation of unknown anion(s) inside the clear cell during MCh stimulation. While the decrease in [K]i and [Cl]i may be instrumental in facilitating influx of ions via Na−K−2Cl cotransporters, the functional significance of MCh-induced cell shrinkage remains unknown.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1424
    Keywords: eccrine ; sweat gland ; cell volume ; cholinergic ; Ca ; potassium ; chloride ; channels ; quinidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The goal of the present study was to elucidate the ionic mechanisms by which cholinergic stimulation induces cell shrinkage in eccrine clear cells. Dissociated Rhesus monkey eccrine sweat clear cells were prepared by collagenase digestion of freshly isolated secretory coils and immobilized on a glass slide in a perfusion chamber at 30°C. The cell was visualized by light microscopy with differential interference contract (DIC) and was recorded with a video system (15,000× total magnification). The cell volume was calculated from the maximal cross section of the cell. Methacholine (MCh)-induced cell shrinkage, which was as much as 30% of resting cell volume, was dose dependent and pharmacologically specific. MCh-induced cell shrinkage was persistent in some cells but tended to partially wane with time in others. MCh-induced cell shrinkage was dependent on the chemical potential gradient for KCl, i.e., increasing [K] in the bath ([K] o ) from 5 to 120mm caused MCh to induce cell swelling, whereas removing [Cl] o at 120mm K partially restored the MCh-induced cell shrinkage. The interpolated null [K] o (medium [K] where the cell volume did not change by MCh) of 71mm agreed with the predicted [K] o,null. MCh-induced cell shrinkage was inhibited completely by 1mm quinidine (K-channel blocker) and partially by 1mm diphenylamine-2-carboxylic acid (DPC, a Cl-channel blocker), but not by 0.1mm ouabain or 0.1mm bumetanide, suggesting that MCh-induced cell shrinkage may be due to activation of both K and Cl channels with the resultant net KCl efflux down the chemical potential gradient. That Ca/calmodulin may be involved in cholinergic regulation of Cl and K channels is suggested because 10 μm ionomycin also induced cell shrinkage, MCh failed to induce cell shrinkage in a Ca-free medium after the endogenous Ca store was depleted, and (6-aminohexyl)-5-chlorol-naphthalenesulfonamide (W-7, a putative inhibitor of calmodulin) also inhibited MCh-induced cell shrinkage in a reversible manner.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0533
    Keywords: Choroid plexus papilloma (CPP) ; Malignant DCPP ; Choroid plexus endothelium ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A disseminated choroid plexus papilloma (DCPP) with a malignant change in the cervico-spinal leptomeninges observed 4 years after the removal of choroid plexus papilloma (CPP), originating from the fourth ventricle, was studied under the electron microscope. Although the ultrastructure of intracranial CPP has been reported by several authors, there are just a few reports on DCPP. The present tumor was ultrastructurally very similar to normal choroid plexus, but the lack of the capillary fenestration and of invaginations of the epithelial basal plasmalemmas suggested that the epithelium was deprived of secretory function.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 62 (1984), S. 225-229 
    ISSN: 1432-0533
    Keywords: Dandy-Walker syndrome ; Dandy-Walker cyst ; Ependymal cell ; Ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the wall of the Dandy-Walker cyst has been described rarely. A boy aged 2 years was confirmed clinically, neuroradiologically, and operatively as having a Dandy-Walker cyst in the posterior fossa. The cyst wall obtained during surgery consisted of an outer arachnoid cell layer, intermediate interwoven neuroglial strands, and an inner layer of cells which lacked the characteristic appearance of ependyma. An unusual finding was a small, buried island of ependymal cells in the intermediate layer of the neuroglial tissue. Ultrastructural study of the cyst wall provides a better understanding of the pathogenesis of the Dandy-Walker syndrome.
    Type of Medium: Electronic Resource
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