Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1573-4927
    Keywords: major urinary proteins ; rate of synthesis ; androgen regulation ; mouse liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract By labeling liver protein in vivo with [3H]leucine, the relative biosynthetic rate has been measured for the major urinary proteins (MUPs), three closely related, androgen-regulated proteins that are synthesized in mouse liver, secreted into the bloodstream, and excreted into the urine. In livers from females of strain C57BL/6J, total MUP synthesis represents about 0.6–0.9% of the total protein synthesis; in males and testosterone-treated females of the same strain, synthesis increases to about 3.5–4.0% of the total. This 4-to 6-fold induction of total MUP synthesis is similar to the androgen-mediated increase in MUP-specific messenger RNA reported by others, and indicates that the previously observed 20- to 25-fold induction of total MUP excretion into urine is generated partly at the posttranslational level. By measuring the ratio of synthesis of the individual MUPs, it was determined that the testosterone-mediated change in the relative levels of the MUPs in urine reflects a similar change in the pattern of MUP synthesis, indicating that the posttranslational processes operate on the quantity, and not the nature, of MUPs excreted. A survey of seven inbred mouse strains revealed polymorphism for the rate of total MUP synthesis in untreated females. Two classes could be distinguished on the basis of a 3- to 5-fold difference in the rate. This variation does not correlate with variation at Mup-a, a locus that controls the ratio of the three MUPs in urine from androgen-induced mice. These findings are consistent with the notion that MUP expression is controlled by a variety of independently assorting genes.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1573-4927
    Keywords: major urinary proteins ; genetic variation ; mouse genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A two- to fourfold difference in the relative rate of total major urinary protein (MUP) synthesis between C57BL/6J and C3H/HeJ female mice has been analyzed at the genetic and molecular levels. The C57BL/6J phenotype is dominant in F1 female progeny of a cross between the two strains. Quantitation of MUP mRNA levels indicates that the rate of synthesis variation does not reflect a change in the concentration of total MUP mRNA. In recombinant inbred strains derived from C57BL/6J and C3H/HeJ progenitors, the rate of synthesis difference segregates as a single genetic determinant that is not linked to the Mup-a locus on chromosome 4. The results suggest an unlinked locus that acts to alter total MUP synthesis without altering total MUP mRNA levels. Two models are proposed to describe the action of this locus, both of which imply some sort of posttranscriptional control of MUP synthesis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1573-4927
    Keywords: major urinary proteins ; genetic variation ; mouse genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A two- to fourfold difference in the relative rate of total major urinary protein (MUP) synthesis between C57BL/6J and C3H/HeJ female mice has been analyzed at the genetic and molecular levels. The C57BL/6J phenotype is dominant in F1 female progeny of a cross between the two strains. Quantitation of MUP mRNA levels indicates that the rate of synthesis variation does not reflect a change in the concentration of total MUP mRNA. In recombinant inbred strains derived from C57BL/6J and C3H/HeJ progenitors, the rate of synthesis difference segregates as a single genetic determinant that is not linked to theMup-a locus on chromosome 4. The results suggest an unlinked locus that acts to alter total MUP synthesis without altering total MUP mRNA levels. Two models are proposed to describe the action of this locus, both of which imply some sort of posttranscriptional control of MUP synthesis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...