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  • fatty acid synthesis  (1)
  • oxidative and respiro-fermentative metabolism  (1)
  • Cryptococcus curvatus
  • Catharanthus roseus
  • Lipophilic amines distribution
  • Wiley-Blackwell  (2)
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  • 1
    ISSN: 0006-3592
    Keywords: high-cell density cultures ; recycle fermentor ; Schizosaccharomyces pombe ; oxidative and respiro-fermentative metabolism ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A strain of the fission yeast Schizosaccharomyces pombe was aerobically grown in a cell-recycle fermentor under various operating conditions, i.e., different bleeding rates and various separate feed rates of glucose and basal medium. Carbon and energy balances were analyzed during steady-state culture regimes, allowing growth yields and maintenance coefficients to be determined under glucose-limited and glucose-excess environments. Special attention was given to the metabolic shift from purely oxidative to respirofermentative glucose catabolism resulting from a change in the growth-limiting factor. No maintenance requirements for the carbon source and for energy were observed during glucose-limited culture regimes and oxidative catabolism. Under glucose excess and respirofermentative metabolism, the mG coefficient was shown to be growth-linked, whereas the enhancement of the apparent me coefficient observed for increased residual glucose concentrations could be assigned to a decline in the ATP yield. © 1993 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-3592
    Keywords: fatty acid synthesis ; yeast ; Rhodotorula glutinis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In nitrogen-limited media, growth and fatty acid formation by the oleaginous yeast Rhodotorula glutinis, i.e., yield and fatty acid cell content, have been characterized regarding carbon and nitrogen availabilities. It was shown that the formation of fatty acid free biomass was limited by nitrogen availability, whereas the fatty acid production was directly dependent on the consumed C/N ratio. According to these observations, the fraction of substrate consumed for fatty acid synthesis was estimated by using a simple method based on the actual yields, i.e., the mass of carbon source strictly converted into fatty acids and fatty acid free biomass. From these results, relationships were established allowing to predict in a simple and performing manner the maximal attainable fatty acid cell content and yield from the available carbon and nitrogen. These relationships were validated by using experimental data obtained by various authors with different yeast strains, and the proposed method was compared to the energetic and mass balance method previously described. © 1993 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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