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  • phorbol ester  (1)
  • Wiley-Blackwell  (1)
  • International Union of Crystallography (IUCr)
  • Munksgaard International Publishers
  • 1
    ISSN: 0730-2312
    Keywords: rat protein kinase C ; recombinant baculovirus ; antisera ; phorbol ester ; isoenzymes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Expression of rat protein kinase C-δ (PKC-δ ) and PKC-ξ in insect cells using recombinant baculovirus resulted in the production of proteins with a molecular size of approximately 76 kD and 78 kD, respectively, as determined by immunoblotting with subtype-specific antisera. Although the PKC-ξ cDNA encoded for 592 amino acids, a 76 kD protein was also generated by in vitro transcription/translation. Extracts of cells expressing PKC-δ were able to bind phorbol ester to levels comparable to extracts of cells expressing PKC-α. No phorbol ester binding was, however, detected in insect cell extracts expressing PKC-ξ. However, similar levels of protein kinase activity were detected in lysates of cells expressing PKC-δ or PKC-ξ when protamine sulfate was used as exogenous substrate. Compared to protamine sulfate, both, myelin basic protein (MBP) or histone, were poor substrates for PKC-δ and PKC-ξ. In contrast to PKC-ξ, the PKC-δ enzyme activity phosphorylated MBP or histone in a phosphatidylserine-(PS)/diacylglycerol(DG)-dependent manner, albeit not to the same extent as PKC-α. Lack of stimulation of the enzyme activity of PKC-ξ by PS/DG, was confirmed by endogenous phosphorylation of insect cell proteins by PKC-ξ, whereas several insect cell proteins were phosphorylated by PKC-δ in a PS/DG-dependent manner, including a protein of 78 kD.Our data demonstrate that the 76 kD PKC-ξ, in contrast to PKC-δ, is unable to bind phorbol esters and displays a protein kinase activity that is independent of PS of PS/DG. In addition, staurosporine was about 2-4 order of magnitudes less effective in inhibiting the protein kinase activities of PKC-δ and PKC-δ when compared to PKC-ξ.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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