characterisation of uromucoid
quantitative determinnation of uromucoid
Springer Online Journal Archives 1860-2000
Summary Uromucoid, the urinary glycoprotein, consists of an immunoelectrophoretically distinct low molecular weight protein and a macromolecular component that can be precipitated with sodium chloride. Uromucoid purified by salt precipitation had chemical and physico-chemical properties similar to those reported previously. The high molecular weight fragment gives a precipitin line in a ring test using an anti-uromucoid serum, but there is a visible reaction by the Ouchterlony technique only after it has been cleaved with sodium dodecyl sulfate. The molecules obtained by the latter method are related immunologically to native uromucoid and are completely identical to the urinary low molecular weight protein. A method is described in detail for the quantitative determination of uromucoid in human urine by single radial immunodiffusion and electroimmunoassay.
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