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  • DKFZ Publication Database  (4)
  • serology  (4)
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  • DKFZ Publication Database  (4)
  • 1
    Keywords: POPULATION ; INFECTION ; BIOMARKERS ; HEALTH ; NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; HELICOBACTER-PYLORI ; METAANALYSIS ; INTERLEUKIN-8 ; serology ; CHINESE MEN
    Abstract: PURPOSE: Chronic inflammation has been hypothesized to play a significant role in the aetiology of cancer, including gastric cancer. In the present study, we sought to examine pre-diagnostic systemic cytokine levels in plasma, which can be seen as markers of aggregate inflammation, and risk of distal gastric cancer in a case-control study nested within the prospective Shanghai Men's Health Study. METHODS: Circulating levels of eight inflammation-related cytokines were measured in the plasma collected at baseline for 180 incident cases of distal gastric cancer and 358 matched controls. Helicobacter pylori sero-positivity was assessed using multiplex serology. Conditional logistic regression was used to calculate odds ratios (ORs) and 95 % confidence intervals (CI). RESULTS: Individuals with IL-8 levels above the lowest quartile were at twofold increased odds of gastric cancer [OR 1.91 (95 % CI 1.05-3.46), OR 2.10 (95 % CI 1.19-3.74), and OR 2.30 (95 % CI 1.26-4.19), for the second through fourth quartiles, respectively]. While there were suggestions of an increase in risk with increased level of many of the other cytokines measured (IL-1beta, IL-2, IL-4, IL-6, IL-10, TNF-alpha, and IFN-gamma), no significant associations were found at the p 〈 0.05 level. Infection with CagA-positive H. pylori did not modify these associations. CONCLUSIONS: In a population with high gastric cancer incidence and high H. pylori prevalence, increased circulating levels of IL-8 may indicate increased risk of gastric cancer. These findings add to our understanding of the disease and further efforts to uncover biomarkers of disease risk.
    Type of Publication: Journal article published
    PubMed ID: 24052422
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  • 2
    Keywords: CANCER ; EPIDEMIOLOGY ; HEPATOCELLULAR-CARCINOMA ; PROTEINS ; INFECTION ; IMPACT ; ASSOCIATION ; antibodies ; PREVALENCE ; glutathione-S-transferase ; ONCOLOGY ; ASSOCIATIONS ; serology ; Mongolia ; POLYOMAVIRUSES
    Abstract: Background: To establish antibody analysis from dried blood spots (DBS) on filter paper for seroepidemiologic infection and cancer association studies, we analyzed data from a population-based study in Mongolia. Methods: Using multiplex serology, we analyzed 985 paired DBS and serum samples from the same donors for antibodies to 12 different proteins from four groups of infectious agents: human papillomaviruses (HPV), Helicobacter pylori (H. pylori), hepatitis C virus (HCV), and JC polyomavirus (JCV). Results: Quantitative antibody reactivities in serum and DES showed good correlation, with median correlation coefficients (Pearson R-2) of 0.88 (range, 0.80-0.90) for high-titer (i.e., H. pylori, HCV, JCV) and 0.79 (range, 0.72-0.85) for low-titer antibodies (i.e., HPV). For high-titer antibodies, serum and DBS data were comparable (median slope of linear trend line, 1.14; range, 1.09-1.21), whereas for low-titer antibodies, DBS reactivities were lower than in serum (median slope, 0.54; range, 0.50-0.80). By extrapolating seropositivity cutoff points previously defined for serum to DBS, we found high agreement (〉89% for all antigens) of dichotomized DBS and serum results and median kappa values for high- and low-titer antibodies of 0.86 and 0.78 (range, 0.78-0.92 and 0.55-0.86), respectively. Epidemiologic associations with known risk factors for HPV antibodies were as strong for DBS as for serum. Conclusions: DBS provide a reliable alternative to serum or plasma for detection of antibodies against various pathogens by multiplex serology. Impact: DBS do not require blood centrifugation and allow storage and shipment at ambient temperature, thus facilitating field work for seroepidemiologic studies especially in environments with limited technical infrastructure.
    Type of Publication: Journal article published
    PubMed ID: 22147363
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  • 3
    Keywords: Helicobacter pylori ; serology ; microbiology ; HELICOBACTER-PYLORI ; PATTERN ; PATTERNS ; antibodies ; antibody
    Type of Publication: Meeting abstract published
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  • 4
    Keywords: EXPRESSION ; DIAGNOSIS ; TYPE-1 ; IDENTIFICATION ; PRIMARY SCLEROSING CHOLANGITIS ; FEATURES ; HELICOBACTER-PYLORI INFECTION ; serology ; MOLECULAR MIMICRY ; CARBONIC-ANHYDRASE
    Abstract: Autoimmune pancreatitis (AIP) is defined by characteristic lymphoplasmacytic infiltrate, ductal strictures and a pancreatic enlargement or mass that can mimic pancreatic cancer (PaCa). The distinction between this benign disease and pancreatic cancer can be challenging. However, an accurate diagnosis may pre-empt the misdiagnosis of cancer, allowing the appropriate medical treatment of AIP and, consequently, decreasing the number of unnecessary pancreatic resections. Mass spectrometry (MS) and two-dimensional differential gel electrophoresis (2D-DIGE) have been applied to analyse serum protein alterations associated with AIP and PaCa, and to identify protein signatures indicative of the diseases. Patients' sera were immunodepleted from the 20 most prominent serum proteins prior to further 2D-DIGE and image analysis. The identity of the most-discriminatory proteins detected, was performed by MS and ELISAs were applied to confirm their expression. Serum profiling data analysis with 2D-DIGE revealed 39 protein peaks able to discriminate between AIP and PaCa. Proteins were purified and further analysed by MALDI-TOF-MS. Peptide mass fingerprinting led to identification of eleven proteins. Among them apolipoprotein A-I, apolipoprotein A-II, transthyretin, and tetranectin were identified and found as 3.0-, 3.5-, 2-, and 1.6-fold decreased in PaCa sera, respectively, whereas haptoglobin and apolipoprotein E were found to be 3.8- and 1.6-fold elevated in PaCa sera. With the exception of haptoglobin the ELISA results of the identified proteins confirmed the 2D-DIGE image analysis characteristics. Integration of the identified serum proteins as AIP markers may have considerable potential to provide additional information for the diagnosis of AIP to choose the appropriate treatment.
    Type of Publication: Journal article published
    PubMed ID: 24349355
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