Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (1)
  • Key words Genetically engineered bacteria  (1)
  • 1
    ISSN: 1432-0789
    Keywords: Key words Genetically engineered bacteria ; Soil invertebrates ; Leaf litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven bacterial strains, most of them bearing natural or recombinant plasmids, were introduced in oak leaf litter or soddy-podzolic soil. In these substrata, which contained litter-dwelling diplopods and isopods, or endogenic earthworms, bacteria survival was followed. In the absence of the animals, the numbers of introduced strains gradually decreased. In the presence of the animals, plasmid-bearing strains of Pseudomonas putida survived at 105–107 CFUs g–1 up to 1.5 months in both leaves and soil. The total numbers of bacteria found in excrements from the soil macrofauna were 5–15 times higher than in the food. The numbers of P. putida in the excrements were equal to or higher than in the food. The numbers of Pseudomonas stutzeri JM302 (pLV1013) and Azospirillum brasiliense ATCC29710 (pFACII) in the excrements were always 2–10 times lower than in the food. The digestive fluid taken from the middle part of the gut of the diplopod Pachyiulus flavipes showed a strong antibacterial activity. Those bacteria with lower survival in the gut appeared to be more sensitive to digestion by the midgut fluid. In contrast, the hindgut fluid did not suppress the viability of P. stutzeri JM302 (pLV1013). We postulate that the introduced bacteria partially survive the midgut passage and then multiply with a high growth rate in the hindgut of the animals. The environmental consequences of the interactions between soil invertebrates and the released bacteria are discussed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...