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  • 1
    Online Resource
    Online Resource
    New York, NY : Springer New York | New York, NY : Springer New York
    Subject(s): Life sciences ; Biochemistry ; Life sciences ; Biochemistry, general
    In: Springer eBooks
    Type of Medium: Online Resource
    Pages: XII, 373 p. 79 illus., 64 illus. in color. , online resource.
    Edition: 2nd ed. 2016.
    ISBN: 9781493937714
    Series Statement: Methods in Molecular Biology, 1451
    Language: English
    Note: Chemical Screening in Zebrafish -- TALEN-Mediated Mutagenesis and Genome Editing -- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome -- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System -- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish -- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase -- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines -- Construction of the Inbred Strain -- Zebrafish as a Model for the Study of Solid Malignancies -- Melanoma Regression and Recurrence in Zebrafish -- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model -- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models -- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos -- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae -- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish -- Studying Lipid Metabolism and Transport During Zebrafish Development -- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish -- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy -- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain -- Quantifying Aggressive Behaviour in Zebrafish -- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish -- A Practical Guide to Light Sheet Microscopy -- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish -- Fiber Optic-Based Photostimulation of Larval Zebrafish -- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.
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  • 2
    Subject(s): Biological models. ; Biology Technique. ; Biological Models. ; Biological Techniques.
    In: Springer Nature eBook
    Description / Table of Contents: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
    Type of Medium: Online Resource
    Pages: XIV, 360 p. 94 illus., 85 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071634011
    Series Statement: Methods in Molecular Biology, 2707
    Language: English
    Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.
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