Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • DKFZ Publication Database  (4)
  • CELL  (4)
  • FLUORESCENCE  (3)
  • imaging  (3)
  • 1
    Keywords: CELL ; Germany ; MICROSCOPY ; imaging ; SAMPLES ; RESOLUTION ; MARKER ; MARKERS ; ENDOPLASMIC-RETICULUM ; methods ; STIMULATED-EMISSION ; DEPLETION ; subdiffraction ; FLUORESCENT PROTEIN
    Abstract: We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers
    Type of Publication: Journal article published
    PubMed ID: 16896340
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: CELLS ; CELL ; Germany ; MICROSCOPY ; imaging ; SYSTEM ; RESOLUTION ; LOCALIZATION ; MITOCHONDRIA ; FLUORESCENCE ; LIGHT ; 2-PHOTON EXCITATION ; FLUORESCENCE MICROSCOPY ; methods ; DIFFRACTION RESOLUTION LIMIT ; STIMULATED-EMISSION ; LIGHT-MICROSCOPY ; ENGLAND ; IMPROVEMENT ; DEPLETION FLUORESCENCE MICROSCOPY ; ORGANELLES ; ORGANELLE ; OPTICAL RECONSTRUCTION MICROSCOPY ; NANOSCOPY
    Abstract: The resolution of any linear imaging system is given by its point spread function (PSF) that quantifies the blur of an object point in the image. The sharper the PSF, the better the resolution is. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot, which extends over at least half the wavelength of light (lambda= 400 - 700 nm) in the focal plane and 〉lambda along the optical axis (z). Although concepts have been developed to sharpen the focal spot both laterally and axially, none of them has reached their ultimate goal: a spherical spot that can be arbitrarily downscaled in size. Here we introduce a fluorescence microscope that creates nearly spherical focal spots of 40 - 45 nm (lambda/16) in diameter. Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles
    Type of Publication: Journal article published
    PubMed ID: 18488034
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: CELLS ; CELL ; Germany ; MICROSCOPY ; imaging ; SUPPORT ; SYSTEMS ; SAMPLE ; SAMPLES ; RESOLUTION ; SUFFICIENT ; FREQUENCY ; FIELD ; FREQUENCIES ; DISPLAY ; REGION ; LIVE CELLS ; FLUORESCENCE ; max ; SECTIONS ; LIGHT ; 2-PHOTON EXCITATION ; 3-DIMENSIONAL TRANSFER-FUNCTIONS ; AXIAL RESOLUTION INCREASE ; COHERENT USE ; CONFOCAL MICROSCOPES ; ELECTROMAGNETIC DIFFRACTION ; FLUORESCENCE MICROSCOPY ; IMAGE-RESTORATION ; OPPOSING LENSES ; three-dimensional imaging
    Abstract: Although the addition of just the excitation light field at the focus, or of just the fluorescence field at the detector is sufficient for a three-to fivefold resolution increase in 4Pi-.uorescence microscopy, substantial improvements of its optical properties are achieved by exploiting both effects simultaneously. They encompass not only an additional expansion of the optical bandwidth, but also an amplified transfer of the newly gained spatial frequencies to the image. Here we report on the realization and the imaging properties of this 4Pi microscopy mode of type C that also is the far-field microscope with the hitherto largest aperture. We show that in conjunction with two-photon excitation, the resulting optical transfer function displays a sevenfold improvement of axial three-dimensional resolution over confocal microscopy in aqueous samples, and more importantly, a marked transfer of all frequencies within its inner region of support. The latter is present also without the confocal pinhole. Thus, linear image deconvolution is possible both for confocalized and nonconfocalized live-cell 4Pi imaging. Realized in a state-of-the-art scanning microscope, this approach enables robust three-dimensional imaging of fixed and live cells at; 80 nm axial resolution
    Type of Publication: Journal article published
    PubMed ID: 15377532
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: CELLS ; CELL ; MICROSCOPY ; PROTEIN ; PROTEINS ; RESOLUTION ; DYNAMICS ; BIOLOGY ; CYCLE ; SIGNAL ; MITOCHONDRIA ; FLUORESCENCE ; GREEN FLUORESCENT PROTEIN ; FEASIBILITY ; INTERFERENCE ; AXIAL RESOLUTION INCREASE ; COHERENT USE ; FLUORESCENCE MICROSCOPY ; OPPOSING LENSES ; microtubule ; 4PI-CONFOCAL MICROSCOPY ; confocal ; EXCITATION ; multiphoton ; two-photon ; FLUORESCENT PROTEIN ; MOLECULAR-SPECTROSCOPY ; NANOMETER ; PtK2 ; QDot
    Abstract: The most prominent restrictions of fluorescence microscopy are the limited resolution and the finite signal. Established conventional, confocal, and multiphoton microscopes resolve at best similar to 200 nm in the focal plane and only 〉= 500 nm in depth. Additionally, organic fluorophores and fluorescent proteins are bleached after 10(4)-10(5) excitation cycles. To overcome these restrictions, we synergistically combine the 3- to 7-fold improved axial resolution of 4Pi microscopy with the greatly enhanced photostability of semiconductor quantum dots. Co-localization studies of immunolabeled microtubules and mitochondria, demonstrate the feasibility of this approach for routine biological measurements. In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail. (c) 2006 Elsevier Inc. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 17045487
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...