Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • DKFZ Publication Database  (1)
  • 1
    Keywords: CANCER ; EXPRESSION ; GROWTH ; GROWTH-FACTOR ; SURVIVAL ; tumor ; carcinoma ; Germany ; MODEL ; SYSTEM ; HEPATOCELLULAR-CARCINOMA ; liver ; TUMORS ; MICE ; RESPONSES ; TRANSPLANTATION ; INDUCTION ; ANTIGEN ; DENDRITIC CELLS ; TOLERANCE ; BONE-MARROW ; MOUSE ; hepatocellular carcinoma ; PROMOTER ; PEPTIDES ; ONCOGENE ; NETHERLANDS ; IMMUNOTHERAPY ; MOUSE MODEL ; MELANOMA PATIENTS ; FAILURE ; albumin ; IMMUNIZATION ; T-CELL TOLERANCE ; IMMUNE-SYSTEM ; therapeutic ; Nodules ; Inducible hepatoma model ; LYMPHOCYTE RESPONSES ; Tumor tolerance
    Abstract: Tumors often induce tolerance in the immune system, which may contribute to the limited success of clinical vaccination against tumors. In order to develop strategies for overcoming tumor tolerance we have developed an inducible mouse model of autochthonus hepatocellular carcinoma growth, which relates more closely to the clinical situation than transplantation tumors. These so-called AST mice harbour a construct consisting of the hepatocyte-specific albumin promoter, a loxP flanked stop-cassette, and the oncogene SV40 large T antigen (Tag). By intravenous application of an adenovirus encoding Cre recombinase the stop cassette was excised, thereby inducing Tag expression and formation of hepatoma nodules in a dose-dependent fashion in about 3 months. Non-induced AST mice showed tumor tolerance, as demonstrated by the failure to reject Tag-positive transplantation tumors and the inability to mount CTL following Tag immunization. Dendritic cell-based immunization with an agonist Tag peptide was able to overcome tolerance and resulted in marked CTL activity against naturally occurring Tag epitopes. importantly, vaccination with the agonist peptide prevented growth of the autochthonous liver tumors and significantly prolonged survival of the animals. Our findings demonstrate that agonist peptides can be used in immunization protocols for breaking of tolerance and induction of CTL that mediate effective anti-tumor responses. In addition, the inducible hepatoma model described here can be used for the design of therapeutic strategies against hepatocellular carcinoma. (C) 2009 Elsevier B.V. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 19428549
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...