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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Human Movement Science 7 (1988), S. 365-406 
    ISSN: 0167-9457
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Sports Science
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0584
    Keywords: Acute lymphoblastic leukemia BCR-ABL rearrangment ; Minimal residual diasease Polymerase chain reaction ; Autologous BMT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Residual leukemic cells are detectable at frequencies as low as 1 in 106 normal cells in patients with Philadelphia chromosome/BCR-ABL-positive leukemias in complete remission (CR) using reverse-transcriptase polymerase chain reaction (RT-PCR) with specific nested primers. The level of minimal residual disease (MRD) in the bone marrow (BM) and the peripheral blood (PB) may favor one of the two as the source for an autologous graft. In order to quantify MRD with RT-PCR we analyzed patients ficolled cells after limiting logarithmic dilutions in normal ficolled buffy-coat cells. In six patients with BCR-ABL-pos ALL who were in CR by conventional criteria (5 in CR1 and 1 in CR2), we studied a total of nine paired BM and PB samples prior to scheduled ABMT. A positive RT-PCR signals was detectable in all samples up to dilutions ranging from 1∶101 to 1∶103 in PB, and at higher titers ranging from 1∶103 to 1∶105 in the BM. The BM titers exceeded the corresponding PB titers in all nine sample pairs by at least 1 log. The mean difference was 1.55 log (geometric mean,n=9) and is statistically significant (p〈0.03). We conclude that residual leukemia in BCR-ABL-positive ALL preferentially locates in the BM compartment, and we assume that PB may yield autologous grafts with significantly less leukemic contamination.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1437-1596
    Keywords: Livor mortis ; Blanching ; Colorimetry ; Measurement of pressure ; Estimation of time of death ; Totenflecke, Wegdrückbarkeit ; Totenflecke, Farbmessung ; Totenflecke, Druckmessung ; Todeszeitschätzung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Bislang wurde die „Wegdrückbarkeit” der Totenflecken bei der Todeszeitschätzung im wesentlichen nach subjektiven Eindrücken beurteilt-danach, ob die Totenflecke leicht, vollständig, schwer und/oder unvollständig wegdrückbar sind. Ein neues Meßsystem objektiviert die Beziehungen zwischen Kraftaufwand und Farbänderung der livores („Wegdrückbarkeit”) durch digitale Verarbeitung von Meßgrößen. Der Kraftaufwand während des Drucks auf den Totenfleck wird mittels Dehnungsmeßstreifen elektronisch erfaßt. Bei Erreichen definierter Druckstärken (10, 20, 30 bis 100 Newton) löst ein Computerprogramm jeweils eine Farbmessung mit einem handelsüblichen Farbdifferenz-Meßgerät aus. Die Farbmetrik arbeitet nach dem Dreibereichsverfahren. Alle Parameter werden ineinem Meßvorgang erfaßt und über einen Meßkonverter in einen Computer eingelesen. Die Darstellung des Farborts erfolgt im L,a b-System (CIELAB nach DIN 5033, DIN 6174), das der Physiologie des Sehens sehr nahekommt. Außerdem können Farbveränderungen über eine Farbabstandsformel (ΔE) weitgehend unabhängig vom Grundton der Haut analysiert werden. In ersten Ergebnissen von Messungen an Leichen zeigen sich regelhafte postmortale Abläufe der Farbveränderungen von Totenflekken bei zunehmender Druckstärke.
    Notes: Summary Until now, blanching of livor mortis in estimating time of death has generally been assessed based on subjective impressions, i.e. on whether blanching is visible after the application of pressure. We have developed a measuring system that uses digital processing to objectify the relationship between the pressure applied and blanching of postmortem lividity. The pressure is electronically registered by a strain gauge. At predefined levels (10, 20, 30, up to 100N) the software triggers a color measurement by a commerically available tristimulus colorimeter. All parameters are measured in a single procedure and routed to the computer through a data interface. The pressure-induced color changes in the livor mortis are evaluated according to the L*, a*, b* system (CIE-LAB according to DIN 5033, DIN 6174), which closely approximates the physiology of sight. An additional color spacing formula (AE) allows analysis of color changes irrespective of the basic skin tone. Initial measurements on cadavers showed that application of increasing pressure produced regular courses of color changes in livor mortis.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2018-10-19
    Description: Molecular measurable residual disease (MRD) assessment is not established in approximately 60% of acute myeloid leukemia (AML) patients because of the lack of suitable markers for quantitative real-time polymerase chain reaction. To overcome this limitation, we established an error-corrected next-generation sequencing (NGS) MRD approach that can be applied to any somatic gene mutation. The clinical significance of this approach was evaluated in 116 AML patients undergoing allogeneic hematopoietic cell transplantation (alloHCT) in complete morphologic remission (CR). Targeted resequencing at the time of diagnosis identified a suitable mutation in 93% of the patients, covering 24 different genes. MRD was measured in CR samples from peripheral blood or bone marrow before alloHCT and identified 12 patients with persistence of an ancestral clone (variant allele frequency [VAF] 〉5%). The remaining 96 patients formed the final cohort of which 45% were MRD + (median VAF, 0.33%; range, 0.016%-4.91%). In competing risk analysis, cumulative incidence of relapse (CIR) was higher in MRD + than in MRD – patients (hazard ratio [HR], 5.58; P 〈 .001; 5-year CIR, 66% vs 17%), whereas nonrelapse mortality was not significantly different (HR, 0.60; P = .47). In multivariate analysis, MRD positivity was an independent negative predictor of CIR (HR, 5.68; P 〈 .001), in addition to FLT3 - ITD and NPM1 mutation status at the time of diagnosis, and of overall survival (HR, 3.0; P = .004), in addition to conditioning regimen and TP53 and KRAS mutation status. In conclusion, NGS-based MRD is widely applicable to AML patients, is highly predictive of relapse and survival, and may help refine transplantation and posttransplantation management in AML patients.
    Keywords: Transplantation, Myeloid Neoplasia, Clinical Trials and Observations
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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