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  • 11
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6–99.5% similarity to Methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A few genera of angiosperms are known as ‘resurrection plants’ since their leaves withstand complete desiccation. In many organisms, including some resurrection plants, desiccation tolerance is associated with the accumulation of special carbohydrates. We examined whether this is also true for the two European angiosperm genera of resurrection plants, Ramonda and Haberlea in the Gesneriaceae. Using gas chromatography, non-structural carbohydrates were determined as a percentage of the dry weight in leaves of Ramonda nathaliae subjected to various desiccation regimes. Sucrose was the predominant soluble carbohydrate in all samples, and its level steadily increased from 2 to 10% during desiccation. Starch amounted to ca 2% in control leaves and disappeared completely within 8 days of desiccation. Considerable amounts (1–2.5%) of raffinose and smaller amounts of its precursor galactinol (1-a-galactosyl-myo-inositol) were present in control leaves; these carbohydrates showed only minor changes upon desiccation. Similar results were obtained when excised leaves of Ramonda nathaliae, Ramonda myconi and Haberlea rhodopensis were subjected to desiccation. These data indicate that sucrose accumulation is connected to desiccation tolerance in Gesneriaceae; the presence of raffinose may be a pre-adaptation since this sugar prevents crystallization of sucrose during drying.
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 145 (1996), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Sucrose, glucose and fructose are degraded in the Gram-negative bacterium Zymomonas mobilis via an anaerobic version of the Entner-Doudoroff pathway, to an equimolar mixture of ethanol and carbon dioxide. Sucrose is split extracellularly into glucose and fructose (or levan). The two sugars are transported into the cell via facilitated diffusion (uniport). A periplasmic enzyme, glucose-fructose oxidoreductase, provides the novel compatible solute, sorbitol, to counteract detrimental osmotic stress. Carbon flux and its regulation, and branches into anabolic pathways are discussed together with recent approaches to broaden the substrate range of the bacterium.
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  • 14
    ISSN: 1573-8868
    Keywords: part-and-whole correlation ; ratio correlation ; regression ; Cantor function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Mathematics
    Notes: Abstract Plots of sedimentation rates vs. time span of observation are routinely used to demonstrate that sedimentation rates decrease if one averages over longer time spans. However, these plots are suspect because they plot a variable, time, against its inverse. It has been shown that even random numbers may yield correlation coefficients of 0.7 or higher under these circumstances. We have circumvented this problem by splitting observed sedimentation rates into time classes and performing regression on the primary variables, thickness and time, separately in each class. An alternative is weighted regression that corrects for the effect of spurious correlation. Regression on the primary variables has been performed on real data from siliciclastic and carbonate rocks. Data were sorted into time classes of 10 −1–10 2 yr, 10 2–10 5 yr, and 10 5–10 8 yr. Sedimentation rates decrease systematically as the time windows increase. The experiment indicates that the decrease of sedimentation rates with increase of time is not simply an effect of the mathematical transformation. It is a physical phenomenon, probably related to the fact that sedimentation is an episodic process and that the sediment record is riddled with hiatuses on all scales.
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  • 15
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1995 (1995), S. 481-486 
    ISSN: 0947-3440
    Keywords: Dyes, fluorescent ; Perylene derivatives ; Heterocyclic rings ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Perylene-3,4:9,10-tetracarboxylic acid 3,4-anhydride-9,10-imides are condensed with primary diamines to a number of new basic chromophores with amidine-imide structures. Lightfast dyes are obtained which exhibit a strong fluorescence in solution. The absorption and fluorescence spectra of the title compounds are bathochromically shifted compared to those of the perylene dyes.
    Additional Material: 2 Ill.
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  • 16
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Recombinant transaldolase from Escherichia coli, an enzyme of the pentose phosphate pathway has been crystallized by the vapor-diffusion method using polyethylene glycol 6000 as precipitant. The crystals are orthorhombic, space group P212121 with cell dimensions a = 68.9, b = 91.3 and c = 130.5 Å and diffract to 2 Å resolution on a conventional X-ray source. The asymmetric unit very likely contains two subunits, corresponding to a packing density of 2.9 Å3 Da−1.
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  • 17
    ISSN: 1432-1335
    Keywords: Key words sICAM-1 ; Lung cancer ; Liver metastasis ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intercellular adhesion molecule-1 (ICAM-1) expression correlates with tumour progression in patients with malignant melanoma or renal cell carcinoma. To assess the value of soluble ICAM-1 (sICAM-1) for lung cancer patients, sICAM-1 was determined by means of an enzyme-linked immunosorbent assay. Sera from 147 patients with lung cancer, from 75 patients with benign lung diseases and from 108 healthy adults were investigated for sICAM-1 expression. Significant differences in sICAM-1 levels were detected in lung cancer patients (387 ± 176 ng/ml) and patients with benign lung diseases (365 ± 110 ng/ml) compared to the group of healthy adults (310 ± 90 ng/ml). There was no difference in sICAM-1 level among the subtypes of lung cancer. Advanced tumour stages and patients with progressive disease tended to be associated with higher sICAM-1 levels, the site of metastasis being relevant for the level attained. Patients with liver metastasis had the highest sICAM-1 levels (547 ± 295 ng/ml) compared to patients with cerebral metastasis (317.8 ± 92.2 ng/ml). An increase of sICAM-1 expression during the progression of the disease coincided with a poorer survival prognosis for the patients compared to patients with stable or falling sICAM-1 levels.
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  • 18
    ISSN: 1432-1335
    Keywords: DNA content ; Squamous cell carcinoma ; Cell line ; Protein synthesis ; Head and neck
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aneuploidy, as abnormal nuclear DNA content, is considered almost positive evidence of malignancy. In this study three diploid and three aneuploid squamous cell carcinoma (SCC) cell lines were examined for DNA content by flow cytometry. The DNA indices of the SCC cell lines were found to range from 1.0 to 2.1. The mitotic activity of the diploid cell lines was 1.6 times higher and the cells were smaller than aneuploid cells. To find a molecular basis for these differences, the pattern of the de-novo synthesized proteins was analyzed by means of [35S]methionine incorporation, electrophoresis, and autoradiography. In all aneuploid SCC cell lines tested in this experiment, the increase of nuclear DNA content is associated with the synthesis of a novel protein with a molecular mass of approximate 55 kDa as well as with altered synthesis rates of two preexisting proteins (50 kDa and 100 kDa). For determination of the amino acid uptake in diploid and aneuploid cells, the accumulation of [35S]methionine was measured as a function of time by liquid scintillation counting. No significant difference was found in the uptake rate between diploid and aneuploid cells with the same protein content. However, discrepancies were revealed when equal numbers of cells with different DNA index were used, suggesting, that protein turnover is different in diploid and aneuploid SCC cells.
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  • 19
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Filmloses Befunden ; PACS ; Teleradiologie ; Bildverteilung ; Key words Filmless reading ; PACS ; Teleradiology ; Digital image distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In healthcare, cost effectiveness as well as the quality of examinations and procedures are subjected to quickly increasing expectations and demands: we like to demonstrate how the resulting challenges and problems can be met with implementation of modern information technology. Analysing the respective demands (pattern of quantities) and choosing the adequate technical solution/ technical approach, we found filmless reading and the usage of digital image distribution to communicate with referring physicians to be cost effective as well as of higher quality. Special attention should be paid to the rigorous maintenance of data security and access. Today’s information technology allows individual adjustment to the respective size and requirements of a radiological department or practice for filmless reading and digital image distribution. Working with the systems as a matter of routine and using all of the expanding technological possibilities, an important improvement of service and quality can be achieved. Amortisation will be obtained despite high investments, due to the subsequent savings in personal- and enterprise costs.
    Notes: Zusammenfassung Der Kostendruck sowie der Anspruch auf Leistung und Qualität im Gesundheitswesen steigen ständig. Am Beispiel einer radiologischen Gemeinschaftspraxis zeigen wir auf, wie diesen Voraussetzungen mit Einsatz moderner Informationstechnologie begegnet werden kann. Nach Analyse des Mengengerüstes und Auswahl der adäquaten technischen Lösung, tragen filmloses Befunden und der Einsatz von digitalen Bildverteilungen an Zuweiser kosteneffektiv zur Qualitätssteigerung bei. Einer konsequenten Einhaltung der Datenschutzbestimmungen und einer Sicherstellung des authentifizierten Datenzugriffes sollte dabei ein Hauptaugenmerk zukommen. Mit Hilfe der vorhandenen Technologien können heute den individuellen Verhältnissen angepaßte Lösungen für digitales Befunden und digitale Bildverteilung erworben werden. Der konsequente Einsatz und die Ausnützung aller Möglichkeiten der Technologie leisten einen wesentlichen Beitrag zur verbesserten Versorgung und Qualitätssicherung. Hohen Anfangsinvestitionen stehen Einsparungen bei Personal- und Betriebskosten entgegen, so daß eine Amortisation zu erreichen ist.
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  • 20
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Transketolase can be used for synthesis of chiral intermediates and carbohydrates. However the enzyme is strongly deactivated by the educts. This deactivation depends on the reactor employed. An enzyme membrane reactor allows the continuous production of L-erythrulose with high conversion and stable operational points. A productivity (space-time yield) of 45g L d was reached.
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