Springer Online Journal Archives 1860-2000
Summary An apparent inhibition of the erythrocyte glutamic oxaloacetic transaminase E-GOT system of Kishi and Folkers has been observed with erythrocytes from individuals on large amounts of B-6. Evidence is presented that this inhibition is an artefact due to excess pyridoxal phosphate reacting with 2,4,dinitrophenylhydrazine. Excess pyridoxal phosphate competes with pyruvic acid for the available 2,4,dinitrophenylhydrazine which results in a product that is not extractable into toluene, therefore, less chromophore is present as more pyridoxal phosphate is available.
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