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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Der Hautarzt 46 (1995), S. 890-891 
    ISSN: 1432-1173
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Protein A from Staphylococcus aureus, characterized by high affinity binding properties for IgG-type antibodies, was labeled with peroxidase to form a stable immuno-histological tracer molecule of comparatively low molecular weight. It has been used for demonstration of antibodies against tissue antigens, in direct and indirect techniques, and the findings were consistent with those in routine immunofluorescence and in staining with peroxidase-coupled anti-IgG. In comparison, the lowest unspecific tissue adsorption and staining was obtained with Protein A-peroxidase in buffer containing glucose, galactose and mannose. The immunohistological preparations were mounted and stored for documentation without apparent loss of staining.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A previous study confirmed the correlation between the vertical diameter of malignant melanomas and prognosis. In 72 of these tumors we examined the pattern and number of reticulin fibers (RF) around and within the melanomas. An increase in vertical diameter was accompanied by an increase of RF within the tumor and a decrease of RF on its edges. The differences were not sufficiently significant to allow firm prognosis.
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  • 4
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Basophilic leucocytes of the blood were collected according to the technique ofShelley andJuhlin. The cells revealedno activity of naphthol-AS-D-chloracetatesterase (technique ofLeder). Mastcells of the skin, however, showed a strong activity, when studied by the Leder-technique. Our results suggest that functional differences exist between basophilic leucocytes (mastcells of the blood) and tissue mastcells.
    Notes: Zusammenfassung Die basophilen Granulocyten, die nach einer Methode vonShelley undJuhlin (1962) [6] angereichert und fixiert worden waren, ließen sich im Gegensatz zu den Mastzellen der Haut mit dem vonLeder (1964) [3] angegebenen Färbeverfahren nicht darstellen. Auch in normalen Blutausstrichen und Ausstrichen von Leukocytenkonzentratwaren diese Zellen mit der eben genannten enzymhistochemischen Färbemethode nicht anfärbbar.
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  • 5
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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  • 6
    ISSN: 1432-1440
    Keywords: Protein A-Peroxidase ; Immunohistologie ; Immunoenzymtechnik ; Antinukleäre Antikörper ; Protein A-peroxidase ; Immunohistology ; Immunoenzyme technique ; Antinuclear antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Protein A from Staphylococcus aureus, a cell wall protein with high affinity binding properties for IgG-type antibodies, has been labeled with peroxidase to form a stable immunohistological tracer molecule of relatively low molecular weight. It has been used for demonstration and titration of antinuclear antibodies in SLE sera on mouse liver sections in an indirect technique. The findings were consistent with those obtained by immunofluorescence and by staining with peroxidase-coupled anti-IgG. In contrast to immunofluorescence, the stained sections could be mounted and stored for documentation. In comparison, unspecific tissue adsorption and staining could be minimized by addition of glucose, galactose, and mannose as well as bovine serum albumine to the buffer containing Protein A-peroxidase.
    Notes: Zusammenfassung Protein A von Staphylococcus aureus, ein Zellwandprotein mit hoher Affinität für Antikörper vom Type IgG, wurde mit Peroxidase markiert. Es entstand ein stabiles Tracer-Molekül von relativ niedrigem Molekulargewicht für den immunohistologischen Nachweis von gewebsgebundenen Antikörpern. In einer indirekten Technik wurden auf Mäuselebergewebe antinukleäre Antikörper aus Seren von Patienten mit Systemischem Lupus Erythematodes nachgewiesen und titriert. Die Befunde stimmten mit denjenigen bei Immunfluoreszenz und bei Immunoenzymtechnik mit Peroxidase-markiertem anti-IgG überein. Im Gegensatz zur Immunfluoreszenz können die Präparate nach der Immunoenzymreaktion zur Dokumentation aufbewahrt werden. Die bei allen Techniken auftretende unspezifische Adsorption von Tracermolekülen im Gewebe konnte durch Zusatz von Glucose, Galactose und Mannose zur Protein A-Peroxidase-Suspension deutlich vermindert werden.
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  • 7
    ISSN: 1432-069X
    Keywords: Histiocytoma ; Fibroma ; Sclerosing angioma ; Histiocytom ; Fibrom ; Sklerosierendes Angiom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Überprüft man in Histiocytomen mit Hilfe des Nachweises der alkalischen Phosphatase den Verlauf der Capillaren, so überrascht die große Zahl der Gefäße. Von einem Zentrum erstrecken sich diese durch das ganze Histiocytom, selbst die über dem Histiocytom unter der Epidermis gelegenen Capillaren sind gewuchert. Histochemische und elektronenmikroskopische Untersuchungen zeigen aber, daß die Mehrzahl der Tumorzellen keine Gefäßzellen sind, sondern Fibroblasten, Histiocyten und Mastzellen. Die jüngeren Fibroblasten gleichen Elementen, die man auch bei der Wundheilung findet und die man als C-Fibroblasten bezeichnet hat.
    Notes: Summary In histiocytomas the alkaline phosphatase technique reveals a surprising number of capillaries. The capillaries branch out from the center to the periphery of the histiocytoma and even the capillaries beneath the epidermis proliferate. The cellular elements outside the capillaries, however, are fibroblasts, histiocytes and mast cells, as is revealed by various histochemical and electron microscopical examinations. Histiocytomas of short duration differ from older histiocytomas in that the former contain fibroblasts similar to those found in healing wounds (C-fibroblasts).
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  • 8
    ISSN: 1432-069X
    Keywords: Psoriasis ; Retinoid ; Epidermal cell proliferation ; Autoradiography ; Psoriasis ; Retinoid ; epidermale Proliferation ; Autoradiographie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 16 Psoriatikern mit generalisierter Psoriasis, die unter oralet Retinoid-Therapie standen (1 mg/kg Körpergewicht täglich), wurden 24h, 48h, 1 Woche und 3 Wochen nach Therapiebeginn autoradiographische Untersuchungen an der befallenen und nicht befallenen Epidermis durchgeführt. Während der ersten Behandlungswoche fanden sich keine signifikanten Veränderungen der zellkinetischen Parameter, doch nach 3wöchiger Therapie kam es zu einer Minderung des gesteigerten 3H-Markierungsindex und zu einer Verkürzung der verlängerten DNS-Synthesezeit; die mittlere Generationszeit blieb weiterhin unbeeinflußt. Diese Befunde zeigen, daß orale Retinoidgaben die veränderte Zellkinetik bie Psoriasis günstig beeinflussen, und zwar sowohl in befallener als auch in nicht befallener Epidermis. Die lange Latenzzeit spricht dafür, daß das Medikament nicht unmittelbar in den Zellcyclus eingreift. Damit wird der gute additive Effekt des Retinoids in der kombinierten klinischen Anwendung mit anderen antipsoriatischen Maßnahmen (Retinoid + Anthralin, Retinoid + PUVA, Retinoid + UVB) verständlich.
    Notes: Summary In 16 patients with generalized psoriasis treated with oral retinoid (Ro 10-9359; 1 mg/kg body weight/day) autoradiographic studies were performed before, and 24h, 48h, 1 week and 3 weeks after beginning of treatment on involved and non involved epidermis. During the first week no significant changes of all cellular parameters were found. However, after 3 weeks of continuing medication the increased 3H-thymidine labelling index was markedly lowered and the prolonged DNA synthesis time was shortened; whereas, the cell cycle time remained unaffected. The findings revealed that oral retinoid has a distinct influence on epidermal cell proliferation towards normalization in both involved and non involved epidermis in psoriasis. The time needed for this effect indicates that the drug does not directly interfere with the cell cycle. It seems reasonable, therefore, to combine oral retinoids as a basic adjuvant drug with other antipsoriatic techniques, such as retinoid + anthralin, retinoid + PUVA, retinoid + UVB.
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  • 9
    ISSN: 1432-069X
    Keywords: Kaposi's sarcoma ; Initial lesion ; HIV infection ; Spindle cells ; AIDS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Kaposi's sarcoma (KS) in human immunodeficiency virus infection (HIV) has become a rather frequent manifestation of the previously rare disease with fatal outcome. Initial lesions of KS were studied by means of histopathology, immunohistology, and electron microscopy in order to define the earliest alterations. The histopathological changes of initial lesions were distinct, consisting of (1) discrete proliferation of capillary vessels, (2) dissection of collagen by proliferating spindle cells which formed slits, (3) atypical spindle cells arranged in an Indian file pattern, and (4) the lack of any inflammatory cellular infiltrate. Double staining with antibodies against vimentin and immunohistochemical markers for endothelial cells revealed that slits forming vimentin-positive spindle cells displayed laminin, factor VIII, and PAL-E. Atypical vimentinpositive spindle cells arranged in an Indian file pattern inconsistently expressed laminin and factor VIII, but not PAL-E. KS cells rarely stained with the lectin UEA I, not even in case of less advanced dedifferentiation. Electron microscopy showed gradual transformation between spindle cells forming slits and those having lost the ability to form incomplete vessel walls. The present findings support the view that KS develops from the endothelial cells of the blood vessels. The proliferation of atypical endothelial cells as early as in initial lesions and the lack of inflammation favors the primary neoplastic genesis of KS.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 202 (1956), S. 556-566 
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Es werden 3 Fälle von benignem Juvenilem Melanom im Sinne von Spitz bei Kindern, davon eins in Art der Fälle von McWorther und Woolner beschrieben, ferner bei einem Zwanzigjährigen ein Übergang zwischen aktiv wachsendem Pigmentzellnaevus und Juvenilem Melanom. Die Differentialdiagnose zum malignen, Melanom, dem Histiozytom und dem Naevoxanthoendotheliom wird erörtert. Das Juvenile Melanom ist eine Sonderform des aktiv wachsenden “Junctional Nevus” und entspricht diesem auch prognostisch.
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