Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • ACI  (1)
  • ANDROGEN  (1)
  • BIOASSAYS  (1)
  • 1
    Keywords: OPTIMIZATION ; RECEPTOR ; CELLS ; IN-VITRO ; CELL ; COMBINATION ; VITRO ; GENE ; GENES ; BIOLOGY ; ASSAY ; CELL-LINE ; LINE ; NETHERLANDS ; RECEPTORS ; ER ; signaling ; SCIENCE ; methods ; ANDROGEN RECEPTOR ; CHEMICALS ; ANTAGONISTS ; ANDROGEN ; androgens ; in vitro ; reporter gene assay ; RANGE ; ANDROGEN-RECEPTOR ; PANEL ; Prevalidation ; ENDOCRINE DISRUPTORS ; REPORTER ; Hershberger assay ; Androgen reporter gene assay ; BIOASSAYS ; HUMAN CELL-LINE
    Abstract: To date there are no validated methods available to test androgenicity or antiandrogenicity in vitro. A problem with testing androgenicity using reporter genes is the possibility by other steroid receptors than androgen receptors to activate the same reporter gene, thereby lowering selectivity. To avoid this we have established a robust and very selective method, the AR CALUX reporter gene assay, to test androgenic and antiandrogenic activity of compounds in vitro. This assay uses a human U2-OS cell line stably transfected with the human androgen receptor and an androgen receptor responsive reporter gene. We optimized protocols to be used in combination with AR CALUX cells and carried out an in house prevalidation. In addition we successfully transferred this assay to another laboratory, leading to comparable test results with a panel of androgen receptor agonists and antagonists. The assay was able to readily rank a range of chemicals on the basis of their EC50 values. The CALUX assay was found to be selective for androgens and seemed not influenced by signaling through other steroid receptors.
    Type of Publication: Journal article published
    PubMed ID: 20438827
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-2102
    Keywords: Schlüsselwörter CTA ; Spiral-CT ; Dissektion ; ACI ; Key words CT angiography ; Spiral CT ; Dissection ; ICA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Purpose: To evaluate the role of CT angiography (CTA) in the diagnosis of dissection of the internal carotid artery (ICA). Methods: In 21 patients who were clinically or sonographically suspected of having a dissection of the ICA, we performed CTA covering the extracranial course of the ICA. Our technique included spiral scanning (Picker PQ 2000), slice thickness 3 mm, index 1.5 mm, pitch factor 1.25, tube voltage 130 kV, amperage 125 mA, i. v. bolus injection of 100 ml nonionic contrast medium, injection rate 4 ml/s and scan delay 15 s. Spiral data were processed using a workstation (Picker Voxel Q) to calculate 3D “angiographic” reconstructions, maximal intensity projections and multiplanar reconstructions. In 20 of the 21 patients transfemoral angiography was performed, and in all patients cw-Doppler ultrasonography of the carotid arteries was performed. Results: Sensitivity of CTA in acute extracranial dissection of the ICA was 100 % (14/14). One patient had a pseudoaneurysm of the ICA, two patients had excessive kinking and one patient showed an atheromatous carotid ulcer. DSA could confirm this in all cases. One intracranial ICA dissection, not covered by the scan field, was missed by CTA. CTA source images demonstrated mural thickening and eccentric luminal narrowing in cases of dissection. 3D reconstructions showed tapering of the ICA. Conclusion: CTA is a reliable tool in the diagnosis of ICA dissection. Further studies comparing CTA, MRI and duplex ultrasound are necessary.
    Notes: Zusammenfassung Ziel der vorliegenden Studie war es, den Stellenwert der CT-Angiographie (CTA) bei der Diagnose von Dissektionen der A. carotis interna (ACI) zu ermitteln. Die Befunde der CTA wurden mit Ergebnissen der digitalen Subtraktionsangiographie (DSA) und der cw-Dopplersonographie (cw-DS) verglichen. Wir untersuchten 21 Patienten mit klinischem oder dopplersonographischem Verdacht auf eine ACI-Dissektion. Bei akuter extrakranieller ACI-Dissektion war die Sensitivität der CTA 100 % (14/14). Eine intrakranielle ACI-Dissektion wurde durch die CTA nicht erfaßt, da sie außerhalb des Scanbereichs lag. Die in der cw-DS unspezifischen pathologischen Befunde konnten mit der CTA geklärt werden. Häufigster Befund in der CTA war eine exzentrische Lumeneinengung und Wandverdickung sowie eine spitzauslaufende Verschmächtigung (sog. tapering) in der dreidimensionalen (3D-) Rekonstruktion. Die CTA ist bei akuter extrakranieller Karotisdissektion eine verläßliche und wenig invasive Untersuchungsmethode. Vergleichende Studien mit CTA, Duplexsonographie und Magnetresonanztomographie (MRT) sollten noch durchgeführt werden.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...