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  • 1
    ISSN: 1617-4623
    Keywords: Key wordsXanthomonas campestris pv. citri ; Bacterio- phage cf ; Pilin ; pilA ; Infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Host factors that are important for infection of Xanthomonas campestris pv. citri by the filamentous bacteriophage cf were investigated by transposon mutagenesis with Tn5tac1. A mutant, XT501, that was resistant to cf infection was recovered, showing that the gene inactivated by the transposon is required for infection by the phage but not for cf replication or assembly. A 1.7-kb SacI-ApaI DNA fragment from XT501 containing the bacterial DNA flanking one end of the transposon was cloned and shown to be required for cf infection. Nucleotide sequence analysis of the 1.7-kb fragment reveals the presence of an ORF that encodes a protein of 146 amino acids. This protein shows 42% identity to the type 4 prepilin encoded by the pilA genes of other bacteria. The pilA gene of X. campestris pv. citri is thus essential for infection by the bacteriophage cf.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1557-1572 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Tower cycling fermentors for the production of single-cell protein from volatile substrates were studied. The mass transfer, mixing and circulation patterns, and residence time distribution (RTD) curves were investigated in these vessels. This study suggests that the tower cycling fermentors for volatile substrates fermentation may improve product yields and at the same time reduce the power consumption, thereby resulting in a significant increase in operating cost savings and capital profits. The results of this research further indicate a future potential for commercial scale tower cycling fermentors.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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