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  • 1
    ISSN: 1573-0972
    Keywords: ERIC-PCR ; PFGE ; S. chincol ; S. weltevreden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolates each from raw vegetables and river water) and S. chincol (15 isolates from poultry) were characterized by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. These isolates originated from a single location in Kajang, Selangor. The results of the PFGE and ERIC-PCR were analysed and comparisons were made using GelCompar software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the S. weltevreden into five clusters and two single isolates and S. chincol into two clusters and two single isolates at a similarity level of 80%, respectively. PFGE produced a single cluster and eight single isolates for S. weltevreden, and one cluster and 11 single isolates for S. chincol at a similarity level of 80% after digestion with the restriction enzyme XbaI, respectively. These results demonstrate that both PFGE and ERIC-PCR are suitable tools for molecular typing of the isolates examined.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2020-11-01
    Description: Introduction Nurse led radial arterial access (RAA) is emerging as an attractive alternative to traditional physician-led access.[1] Methods A multidisciplinary program was developed by the nursing and medical teams in a busy tertiary cardiac centre performing over 3000 interventional cardiology procedures of which 90% of cases are done radially. Phase 1 of the training programme consisted of 50 procedures per nurse performed under direct consultant operator supervision. Phase 2 consisted of routine nurse led radial artery cannulation on unselected patients. In addition 2 sub-studies were performed an audit of patient experience comparing the two nurse practitioners with cardiology trainees. An audit of the time to sheath insertion was carried out. Results Technical success was defined as access within two puncture attempts. In phase 1 of the training two nurses were successful in 84% of cases. In this case cohort there were no identified vascular complications. One patient needed an alternate access route. In Phase 2 311 cases were performed independently on unselected patients. The technical success rate was 86.5% for both nurses in total in this cohort. This included a mixture of both right radial (249 cases) and left radial (25 cases) access. 8 of these were also PPCI cases. No major vascular complications were identified. In cases where no access after two attempts was secured access was gained by a consultant. In 6 cases ultrasound guidance was used by the consultant. A patient discomfort score was recorded immediately via a questionnaire post access procedure. Results were compared with cardiology trainees performing RAA in parallel, demonstrating excellent outcomes. Similarly when comparing difference between patient on table to sheath insertion times between trainees and nurses performing the radial access, nurse radial access had competitive outcomes. Discussion Nurse led RAA procedure is an advanced practice skill which can be adopted by appropriately trained and experienced senior members of the nursing team. The advantages include facilitating flow through the catheter lab, greater advocacy of patients through their clinical pathway and high levels of patient satisfaction. Median Time Funding Acknowledgement Type of funding source: None
    Print ISSN: 0195-668X
    Electronic ISSN: 1522-9645
    Topics: Medicine
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