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  • Electrical activation  (1)
  • 1
    ISSN: 1040-452X
    Keywords: MPF ; Ca2+ ; Electrical activation ; Cattle oocyte ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The influence of number of Ca2+ stimulations on the profile of histone H1 kinase activity in bovine oocytes was investigated. A Ca2+ stimulation consisted of transferring oocytes directly from culture medium to mannitol containing 100 μM Ca2+ and pulsing oocytes with a 0.2 kVcm-1, 20 μsec discharge. One, three, or six Ca2+ stimulations were given, each 22 min apart. Oocytes were frozen from 0 to 8 hr after the first stimulation at indicated time points and assayed for histone H1 kinase activity. H1 kinase activity was quantified using a densitometer and expressed as a percent of activity in nonpulsed metaphase II oocytes. Stimulating oocytes in the absence of Ca2+ in the pulsing medium did not inactivate H1 kinase. In the presence of Ca2+, however, H1 kinase was rapidly inactivated after stimulation. A single stimulation decreased H1 kinase activity to 44% ± 11% of its initial level in 1 hr. However, H1 kinase was dramatically reactivated at 2 hr after the stimulation and reached 122% ± 22% of the initial activity at 6 hr. With three stimulations, basal H1 kinase activity was 21% ± 3% and was obtained in 30 min. H1 kinase reactivation started at 4 hr after the first stimulation and level of activity reached 38% ± 15% at 8 hr. Six stimulations also led to rapid H1 kinase inactivation and to a basal activity of 14% ± 0.4%. With six stimulations, however, basal H1 kinase activity was maintained over at least 8 hr, and no reactivation occurred during this period. Basal H1 kinase activity obtained after six stimulations was similar to that of fertilized oocytes. Immunoprecipitation of p34cdc2 with an anti-cdc2 antibody strongly suggested an identity between histone H1 kinase and maturation-promoting factor. The data indicate that histone H1 kinase activity in oocytes could be regulated by the number of Ca2+ stimulations. A single Ca2+ stimulation led to H1 kinase inactivation, followed by reactivation of the kinase. Increasing the number of Ca2+ stimulations delayed the onset and reduced the extent of H1 kinase reactivation in the first parthenogenetic cell cycle. © 1993 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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