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  • 1
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Simultaneous Functional Magnetic Resonance and Two Dimensional Optical Imaging Spectroscopy -- Neurovascular Coupling Investigated by Simultaneous Optical Coherence Tomography and Electrophysiology -- Studying Adenosine and Cerebral Blood Flow (CBF) Using Pharmacological Methods -- Imaging Vasodynamics in the Awake Mouse Brain with Two-Photon Microscopy -- Neurovascular Coupling in the Deep Brain Using Confocal Fiber-Optic Endomicroscopy -- Non-Invasive Neural Imaging and Tissue Oxygenation in the Visual System -- High-Resolution Wide-Field Optical Imaging of Micro-Vascular Characteristics: From the Neocortex to the Eye -- Optical Intrinsic Signal Imaging for Elucidating Functional Structures in Higher Visual Area -- Closed Cranial Window Applications in the Rat: Studies on Neurovascular Coupling Involving Pial Arterioles and the Glia Limitans -- Multi-Spectral Imaging of Blood Volume, Metabolism, Oximetry and Light Scattering -- Physiological Basis of BOLD fMRI Decreases -- Simultaneous Multi-Wavelength Optical Imaging of Neuronal and Hemodynamic Activity -- Non-Invasive Laser-Induced Photoacoustic Tomography for Functional Imaging -- Neurovascular Coupling in Relation to Cortical Spreading Depression -- Cerebral Blood Flow Imaging with Laser Speckle Contrast Imaging -- Cerebral Monitoring and Surveillance Using High Resolution Functional Optical Imaging
    Abstract: Neurovascular Coupling Methods brings the reader up to date with the current state-of-the-art techniques in measuring blood flow in the brain, with chapters describing different techniques, or combinations of techniques, applied to specific species in either healthy or abnormal brains. Opening with a section on techniques in normal somatosensory processing, the detailed volume continues with parts covering techniques in normal visual processing, epilepsy, and cerebrovascular diseases, such as ischemia, hemorrhage, and spreading depression.℗ As a collection in the popular Neuromethods series, this book contains the kind of thorough description and implementation advice that will lead to successful results in the lab. ℗ Authoritative and practical, Neurovascular Coupling Methods serves as an ideal guide for researchers aiming to further our knowledge of these coupling mechanisms in the hopes of ultimately understanding higher order functions such as language and memory and developing novel brain mapping techniques that can be employed in humans
    Pages: XIX, 332 p. 120 illus., 99 illus. in color. : online resource.
    ISBN: 9781493907243
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  • 2
    Unknown
    Totowa, NJ : Humana Press
    Keywords: Medicine ; Neurosciences ; Psychiatry ; Laboratory animals ; Biomedicine ; Neurosciences ; Psychiatry ; Animal Models ; Springer eBooks
    Description / Table of Contents: Introduction: Binge Eating, Bulimia Nervosa, and Hedonic Overeating -- Binge-Prone vs. Binge-Resistant Rats and Their Concomitant Behavioral Profiles -- Binge Eating in Female Rats Induced by Yo-Yo Dieting and Stress -- Binge-Type Eating Induced by Limited Access to Optional Foods -- Assessment of Stress-Independent Binge-Like Eating Behavior in Mice -- Predicting and Classifying Rats Prone to Overeating Fat -- Modeling Binge Eating in Non-Human Primates -- Psychosocial Stress and Diet History Promote Emotional Feeding in Female Rhesus Monkeys -- Stressful Experiences in Early Life and Subsequent Food Intake -- Sham Feeding in Rats Translates Into Modified Sham Feeding in Women with Bulimia Nervosa and Purging -- Animal Models of Binge Eating Palatable Foods: Emergence of Addiction-Like Behaviors and Brain Changes in the Rat -- Deep Brain Stimulation for the Treatment of Binge Eating: Mechanisms and Preclinical Models -- Saccharin Preference in Rats: Relation to Impulsivity and Drug Abuse -- Food Seeking in Spite of Harmful Consequences -- Introduction: Anorexia and Undereating -- Food Restriction and Reward in Rats -- Activity-Based Anorexia in the Rat -- Food-Anticipatory Activity: Rat Models and Underlying Mechanisms -- Anorexia and Drugs of Abuse Abnormally Suppress Appetite, the Result of a Shared Molecular Signal Foul-Up -- The Anorectic Phenotype of the Anx/anx Mouse is Related to Hypothalamic Dysfunction -- Functional Magnetic Resonance Imaging in Awake Rats: Studies Relevant to Addiction and the Reward Circuitry
    Abstract: The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research.℗ Animal Models of Eating Disorders serves as a collection of detailed techniques contributed by experts in the field who are well-versed in the development and implementation of these models. Since eating disorders are complex and likely due to a combination of environmental, genetic, and social causes, the detailed chapters of this volume have been designed to highlight different contributing factors. Collectively, these chapters give a comprehensive and representative overview of both recently developed and classic methodologies used in the study of eating disorders.℗ Written for the popular Neuromethods series, this work contains the kind of thorough description and implementation advice that promises successful results. ℗ Authoritative and practical, Animal Models of Eating Disorders aims to aid researchers in the use of animal models to assist in their investigation and characterization of the behaviors and neurochemical alterations associated with these devastating disorders
    Pages: XVI, 379 p. 76 illus., 10 illus. in color. : digital.
    ISBN: 9781627031042
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  • 3
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton -- Visualizing In Vitro Trafficking -- Interaction of the Glycocalyx with the Actin Cytoskeleton -- Cytoskeleton Dynamics and Binding Factors -- Principles of Proteomic Approaches to the Cytoskeleton -- Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus -- Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes -- The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases -- Neurofilaments: Properties, Functions, and Regulation -- From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics -- Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton -- Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact -- Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis -- Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System -- Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
    Abstract: The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered.℗ The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system.℗ Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results. ℗ Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton
    Pages: XIV, 359 p. 52 illus., 31 illus. in color. : digital.
    ISBN: 9781627032667
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  • 4
    Keywords: Medicine ; Human Physiology ; Neurosciences ; Biomedicine ; Neurosciences ; Human Physiology ; Springer eBooks
    Description / Table of Contents: Optogenetic Stimulation of Neurons in the Brainstem of Freely Behaving Rats and Mice -- Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex responses in the In Situ Arterially-Perfused Preparation -- Excitatory Responses to Microinjection of Glutamate Depend on Dose not Volume: A Meta-Analysis of Studies in Rat RVLM -- Acute Activation and Inhibition of the Sympathetic Baroreceptor Reflex -- Pharmacological Dissection of G Protein-Mediated Second Messenger Cascades in Neurons -- In Vivo Manipulation of Intracellular Signalling Pathways -- Identification of Spinally Projecting Neurons in the Rostral Ventrolateral Medulla In Vivo -- Microionotophoretic Study of Individual Neurons During Intracellular Recording -- Neurobiotin Electroporation for Combined Structural and Functional Analysis of Neurons in Developing Mouse Brain Slices -- Juxtacellular Neuronal Labelling, Physiological Characterization, and Phenotypic Identification of Single Neurons In Vivo -- Analysis of Sympathetic Nerve Activity Analysis of Sympathetic Nerve Activity -- Insight into Autonomic Nervous System Control of Heart Rate in the Rat Using Analysis of Heart Rate Variability and Baroreflex Sensitivity -- Neurophysiological Recording of the Compound Muscle Action Potential for Motor Unit Number Estimation in Mice -- Animal Models of Neuropathic Pain Due to Nerve Injury -- Detection of Sensitized Nerve Responses: Dorsal Root Reflexes, Live Cell Calcium, and ROS Imaging -- Visual Evoked Potential Recording in Rodents -- The Visual Evoked Potential in Humans -- Improving Motor Activation Patterns after Stroke with Wii-Based Movement Therapy
    Abstract: Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools.℗ In Stimulation and Inhibition of Neurons, experts in the field provide an overview of modern methods for generating lesions as well as for stimulating and inhibiting neural pathways.℗ Many new techniques such as optogenetics and the use of the in situ perfused preparation are examined, while, in other sections, the use and validity of more well-known approaches are reassessed. Written for the Neuromethods series, chapters examine their respective topics thoroughly and include the kind of detail and implementation advice that ensures successful results in the laboratory. ℗ Authoritative and cutting-edge, Stimulation and Inhibition of Neurons serves as an ideal guide for researchers seeking to gain further knowledge of the complex functions of the brain
    Pages: XII, 319 p. 73 illus., 15 illus. in color. : digital.
    ISBN: 9781627032339
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  • 5
    Keywords: Neurosciences ; Laboratory animals ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Fluid Percussion Injury Rodent Model in Preclinical Research on Traumatic Brain Injury -- Focal and Restricted Traumatic Injury Models in the Rodent Brain—Limitations, Possibilities, and Challenges -- A Model for Research on Penetrating Traumatic Brain Injuries -- A Sagittal Plane Rotational Injury Rodent Model for Research on Traumatic Brain Injuries -- Experimental Models of Blast-Induced Neurotrauma -- Utilization of Shock Tubes in Blast Injury Research -- Introduction to Blast in the Context of Blast Induced TBI -- The Clemedson Blast Tube -- Traumatic Peripheral Nerve Injuries – Experimental Models for Repair and Reconstruction -- CNS Regeneration in Nerve Grafts - Practical Aspects of Complete Thoracic Spinal Cord Injury in Rodents -- Experimental Nerve Plexus Root Avulsion and Spinal Cord Implantation -- Surgical Replantation of Avulsed Lumbosacral Ventral Roots and Urodynamic Studies in a Rhesus Macaque (Macaca mulatta) Model of Cauda Equina/Conus Medullaris Injury and Repair -- Secondary Insults in Experimental Traumatic Brain Injury – The Addition of Hypoxia -- A Translational Model of Traumatic Brain Injury: Sheep Impact Acceleration -- In Vitro Trauma – Description of a Cavitation Inducing Injury Model -- Clinically Relevant Outcome Measures for Experimental Traumatic Brain Injury (TBI) Studies -- Concluding Remarks
    Abstract: This volume looks into the need for a variety of experimental models for research on traumatic brain injury (TBI) and peripheral nervous system. It also describes a number of experimental models, such as mechanical devices, that have been developed to model neurotrauma in animal experiments. The overall aim of this book is to explore the variety of models and how they are used in current research. The chapters in this book are organized in four sections and talk about animal models for TBI; animal models for spinal cord or nerve injury; and translational aspects, secondary injuries, in vitro studies, and evaluation of large data sets. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Animal Models of Neurotrauma is a valuable resource for researchers interested in expanding their knowledge and research in this developing field
    Pages: XIII, 302 p. 93 illus., 63 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493997114
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  • 6
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Proteomics ; Neurosciences ; Proteomics ; Springer eBooks
    Description / Table of Contents: Neuroproteomics: The Methods -- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice -- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue -- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics -- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3) -- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip -- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes -- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry -- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons -- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures -- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data -- 2D-DIGE as a Tool in Neuroproteomics -- Neuropeptidomics of the Mammalian Brain -- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides -- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder
    Abstract: This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms
    Pages: XIII, 218 p. 55 illus., 37 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493996629
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  • 7
    Unknown
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume discusses the importance of imaging, fluid, and genetic biomarkers in detecting the earliest pre-symptomatic stages of Alzheimer’s disease (AD). The chapters in this book are separated into five parts. Part one explores the reasons why we need improved approaches for early detection and diagnosis of AD. Part two describes clinical and research techniques for preclinical AD diagnosis. Part three looks at the current methods in use to diagnose AD and how they can also be used for early detection in healthy older individuals. Parts four and five talk about technological advancements in AD diagnosis and ethical considerations in AD research. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Biomarkers for Preclinical Alzheimer’s Disease is a valuable resource for individuals interested in the use of biomarker strategies to improve early AD detection. This book will be of great interest to researchers and clinicians from both academia and industry
    Pages: XIII, 272 p. 39 illus., 33 illus. in color. : online resource.
    ISBN: 9781493976744
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  • 8
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Extracellular and intracellular recordings -- Theory of electrical transfer -- Amplifier -- Salt environment -- Micropipettes -- Spatiotemporal effects of synaptic input -- Perforated patch -- Isolation of synaptic current -- Fast and slow synaptic currents -- Measuring kinetics of synaptic current -- Measuring release probability -- Long-term measurements -- Measuring reversal potentials -- Amplitude -- Pre- and post-synaptic effect -- Run-up and run-down -- Kinetics of synaptic current -- Measurements of a single synapse -- Measurements of silent synapses -- Dendritic patch -- Electrophysiological tag -- In vivo electrophysiology
    Abstract: Electrophysiology is one of the finest approaches capable of detecting and analyzing electrical events in the brain. However, like all other experimental approaches, it has its limitations. This book was created to serve as a guide for those endeavoring to understand the function of electrophysiology and its underlying components: neurons, neural systems, and the brain. It introduces and highlights important topics in the field of electrophysiology, while also extending these topics to practical electrophysiological approaches through the perspective of the two authors. This book is ideal for graduate students or junior postdoctoral fellows interested in starting a journey in synaptic neuroscience
    Pages: XII, 263 p. 89 illus., 46 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493932740
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  • 9
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: In Vivo Whole-Cell Recordings -- Juxtasomal Loose-Patch Recordings in Awake, Head-Fixed Rats to Study the Link between Structure and Function of Individual Neurons -- Studying Sodium Channel Gating In Heterologous Expression Systems -- Elucidating the Link between Structure and Function of Ion Channels and Transporters with Voltage-Clamp and Patch-Clamp Fluorometry -- Dendrites: Recording from Fine Neuronal Structures Using Patch-Clamp and Imaging Techniques -- Patch-clamp Recording from Myelinated Central Axons -- Analysis of Transsynaptic Attentional Neuronal℗ Circuits with Octuple Patch-Clamp Recordings -- Optogenetic Dissection of the Striatal Microcircuitry -- Paired Recordings from Synaptically Coupled Neurones in Acute Neocortical Slices -- Extracting Quantal Properties of Transmission at Central Synapses -- Acousto-optical Scanning-Based High Speed 3D Two-Photon Imaging In Vivo -- Intracellular Voltage-Sensitive Dyes for Studying Dendritic Excitability and Synaptic Integration -- Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels -- Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches -- Creating and Constraining Compartmental Models of Neurons Using Experimental Data
    Abstract: This volume presents current adaptations of the patch-clamp technique to neuroscience. Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical,℗ Advanced℗ Patch-Clamp Analysis for Neuroscientists aims℗ to ensure successful results in the further study of this vital field
    Pages: XII, 350 p. 81 illus., 28 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493934119
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  • 10
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH,℗ and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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